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Plasmid#217479PurposeMammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1bDepositorInsertNier1b
UseTags3xNLS, P2A-EGFP-3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA
Plasmid#216320PurposeSplit luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Luciferase + splice donor site
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-TadA7.10-SpG N aa2-713-InteinN
Plasmid#206967PurposeExpresses TadA7.10 and SpG cas9N by the constitutive CMV promoterDepositorInsertCMV, TadA7.10, SpG N
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-TadA8e-SpG N aa2-713-InteinN
Plasmid#206968PurposeExpresses TadA8e and SpG cas9N by the constitutive CMV promoterDepositorInsertCMV, TadA8e, SpG N
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA-FLAG Chtop-exon minimal CMV pCDNA5
Plasmid#212090PurposeLR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells (BioID vector)DepositorInsertChtop-exon (Chtop Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA-FLAG Kdm1a-exon minimal CMV pCDNA5
Plasmid#212088PurposeLR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells (BioID vector)DepositorInsertKdm1a-exon (Kdm1a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA-FLAG Kdm1a+exon minimal CMV pCDNA5
Plasmid#212089PurposeLR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells (BioID vector)DepositorInsertKdm1a+exon(12nt) (Kdm1a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA-FLAG Chtop+exon minimal CMV pCDNA5
Plasmid#212091PurposeLR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells (BioID vector)DepositorInsertChtop+exon(138nt) (Chtop Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA-FLAG Sap30bp-exon minimal CMV pCDNA5
Plasmid#212092PurposeLR vector for integration of Sap30bp-exon into N2a FRT rtTA3 expression cells (BioID vector)DepositorInsertSap30bp-exon (Sap30bp Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA-FLAG Sap30bp+exon minimal CMV pCDNA5
Plasmid#212093PurposeLR vector for integration of Sap30bp+exon(48nt) into N2a FRT rtTA3 expression cells (BioID vector)DepositorInsertSap30bp+exon(48nt) (Sap30bp Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2
Plasmid#209061PurposeEntry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.DepositorInsertKDM6A
UseGateway vector to be used for lr reactionTagsExpressionMutationPromoterU6Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2
Plasmid#209062PurposeEntry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.DepositorInsertnon-targeting sgRNA (C0111)
UseGateway vector to be used for lr reactionTagsExpressionMutationPromoterU6Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro
Plasmid#210855PurposeLentiviral expression vector encoding FIP200 mutant-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationV44D/V314DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)
Plasmid#208970PurposeRecruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT aa 1-317 RAD23A (delta UBA2 domain)
Plasmid#201465PurposeExpresses a mutant form of human RAD23A that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.DepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsFLAGExpressionMammalianMutationLacks C-terminal residues 318-363PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)
Plasmid#201545PurposeExpresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
UseTagsFLAGExpressionMammalianMutationLacks N-terminal residues 1-79PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)
Plasmid#201548PurposeExpresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
UseTagsFLAGExpressionMammalianMutationLacks C-terminal residues 364-409PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-Δ37, 115-133, 204-217 PPM1H
Plasmid#207344PurposeMammalian expression of HA-Δ37, 115-133, 204-217 PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHAExpressionMammalianMutationΔ37, Δ115-133, Δ204-217PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX
Plasmid#209712PurposeMammailan expression of WDR5, SH2, and HRas separated by P2A.DepositorInsertWDR5-P2A-SH2-P2A-Ras-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-CMV-Cre-U6-sgNotch2#1-U6-sgRb1-U6-sgTrp53-U6-sgRbl2
Plasmid#209069PurposeEntry vector that encodes sgRNAs against mouse Notch2, Rb1, Trp53, Rbl2, and CMV Cre recombinase.DepositorInsertsgRNAs targeting Notch2, Rb1, Trp53, Rbl2 and Cre recombinase
UseGateway vector to be used for lr reactionTagsExpressionMutationPromoterU6Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only