Addgene

Showing: 4761 - 4780 of 150501 results

Results per page:

Sort results by:

pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Plasmid

#179115

Purpose

Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Cas9-GFP_sg_mAMPKa1

Plasmid

#79004

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa1 (Prkaa1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
Twinkle-APEX2-V5

Plasmid

#129705

Purpose

APEX2 fused to the mitochondrial nucleoid protein Twinkle

Depositor

Alice Ting

Article

Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

Insert

Twinkle-APEX2-V5 (TWNK, APEX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Article

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-GAS6-COMP5AP-AviTag-9xHis

Plasmid

#157511

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

GAS6 (GAS6 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Article

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-FOXI1

Plasmid

#153126

Purpose

Expression of Flag tagged human FOXI1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXI1 (FOXI1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-hsFlt3L

Plasmid

#25074

Depositor

Maria Castro

Article

Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

Insert

human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pST44-AP-1 core

Plasmid

#184809

Purpose

Bacterial expression of clathrin adaptor AP-1 core complex

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1

Use

Tags

GST tag and His6

Expression

Bacterial

Mutation

AP1S3: S148C; AP1B1: K359R, E476K

Promoter

T7

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTag-BFP-C-h-Rab11a

Plasmid

#79805

Purpose

Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Use

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
LYSO-LRRK2

Plasmid

#193582

Purpose

Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus

Depositor

Mark Cookson

Article

Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

Leucine-rich repeat kinase 2 (LRRK2 Human)

Use

Tags

LAMTOR1(1-39aa) and 3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINK-Cezanne (OTU, aa 53-446)

Plasmid

#61581

Purpose

Expresses human Cezanne (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne (OTUD7B Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-52 and aa 447-843.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19_HDRT_TRAC_CD19-CAR

Plasmid

#183473

Purpose

Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

Depositor

Dimitrios Wagner

Article

Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

Inserts

TRAC (TRAC Human)
CD19-specific CAR transgene insert
TRAC (TRAC Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-HRE-dUnaG

Plasmid

#124372

Purpose

UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

Depositor

Roland Friedel

Article

Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

Insert

HRE-dUnaG

Use

Lentiviral

Tags

PEST-degron and Myc tag

Expression

Mutation

Promoter

HRE (HIF responsive element 5x + CMV minimal prom…

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR1a-tagRFP-KDEL

Plasmid

#114177

Purpose

Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Gateway entry clone

Tags

KDEL retention signal and signal peptide

Expression

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHD-SPARC2-I-LexA::p65

Plasmid

#133563

Purpose

Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

LexA::p65

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

20X UAS

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetrosuper Myc shRNA

Plasmid

#15662

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Myc shRNA (MYC Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
BCL2A1A

Plasmid

#42399

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BCL2A1A (BCL2A1 Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD3 (OTU, aa 52-209)

Plasmid

#61412

Purpose

Expresses human OTUD3 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 210-398.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-3'3xFlag

Plasmid

#208616

Purpose

Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

Depositor

Quanfu Ma

Type

Empty backbone

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge