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Plasmid#144559PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOSL2 (FOSL2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L
Plasmid#196136PurposeSB vector for the mammalian expression of GADD34-K3LDepositorInsertGADD34 Δ1-240 - P2A - K3L (PPP1R15A vaccinia virus, Human)
UseTagsP2AExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 pegRNA2 with trimmed attP
Plasmid#222347PurposepegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installationDepositorInsertAAVS1 attP-installation pegRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF184
Plasmid#89482PurposeExpress asparaginyl endopeptidase 1 from O. affinis (OaAEP1)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
UseTagsSmt3ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mt-mKeima-P2A-FRB-Fis1
Plasmid#135295Purposestably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cellsDepositorInsertmt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
UseLentiviralTagsExpressionMammalianMutationmt-mKeima is fused with FRB-Fis1 via P2A seqeuncePromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.Luciferase.IRES.GFP
Plasmid#169308PurposeConstitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent proteinDepositorInsertLuciferase
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterSFFVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3B WT
Plasmid#123092PurposeExpresses 3xFLAG-LC3B wild-type in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.2
Plasmid#208101PurposeBiosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179436PurposeDual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertHS4-TRE-cit-HS4-mch (DH)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only