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Showing: 4741 - 4760 of 6515 results

  1. pET151-Vps4(101-437)-Hcp1 K321D

    Plasmid
    #89288
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Use
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 to Asp
    Promoter
    T7
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89288-plasmid-map-sequence-id-175369

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  2. pET151-Vps4(101-437)-Hcp1 R325D

    Plasmid
    #89289
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Use
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Arg-325 to Asp
    Promoter
    T7
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89289-plasmid-map-sequence-id-175352

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  3. pET151-Vps4(101-437)-Hcp1 K321A

    Plasmid
    #87747
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Use
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 to Ala
    Promoter
    T7
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    87747-plasmid-map-sequence-id-175355

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  8. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid
    #101218
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N692A, M694A, Q695A and H698A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101218-plasmid-map-sequence-id-198035

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  9. (SUMO)10-(SIM)5

    Plasmid
    #126866
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126866-plasmid-map-sequence-id-263307

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  14. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid
    #185791
    Purpose
    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker
    Depositor
    Lizhong He
    Article
    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864
    Insert
    RBP-sfGFP(S45C)-ZdK1
    Use
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Ser to Cys mutation at start of GFP, residue 45 i…
    Promoter
    T7
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185791-plasmid-map-sequence-id-367545

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Showing: 4741 - 4760 of 6515 results