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Plasmid#190388PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2600
Plasmid#190387PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2599
Plasmid#190386PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLBT0130
Plasmid#172809PurposeLevel 1 module containing 35S-CTP-mCHERRY-cYFP_P3 FDepositorInsertp35S-5UTMV-CTPSSU-mCHERRY-cYFP3XHA-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0150
Plasmid#172811PurposeLevel 1 module containing 35S-CTP-mCHERRY-cVENUS_P3 FDepositorInsertp35S-5UTMV-CTPSSU-mCHERRY-3XFLAGcVENUS-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0140
Plasmid#172810PurposeLevel 1 module containing 35S-CTP-mCHERRY-nVENUS_P2 FDepositorInsertp35S-5UTMV-CTPSSU-mCHERRY-nVENUS-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0120
Plasmid#172808PurposeLevel 1 module containing 35S-CTP-mCHERRY-nYFP_P2 FDepositorInsertp35S-5UTMV-CTPSSU-mCHERRY-3XFLAGnYFP-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJB1-RPL1B-Cl
Plasmid#160430PurposeExpresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoterDepositorInsertRPL1B
UseTagsmCherryExpressionYeastMutationRemoved introns, shuffled codons to vary nucleoti…PromoterS. cerevisiae GAL1Available sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G06_ZIKV NS4A
Plasmid#145356PurposeCell-free expression vector for Zika virus NS4A protein with C-term mCherryDepositorInsertZIKV NS4A
UseTagsmCherry and 8xHisExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHL391
Plasmid#62821PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1DepositorInsertsgfp
mCherry
UseSynthetic BiologyTagshfp leader, ompC leader, and st7 RBSExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL518
Plasmid#62823PurposeLim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1DepositorInsertsmCherry
gfp
UseSynthetic BiologyTagsm9::ompC leader and micC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
OpenLoopControl
Plasmid#59895Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTRDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPESTExpressionMammalianMutationPromoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 100
Plasmid#52989PurposepLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfpDepositorInsertsOxyS sRNA
fhlA
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutation−309 to +96 bp relative to start codonPromoterpLlac0-1 and pLtetO-1Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 802
Plasmid#52991PurposepLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfpDepositorInsertsOxyS sRNA
ompC
UseSynthetic BiologyTagsGFP and RBS-mCherryExpressionBacterialMutationcontains −81 to +36 bp relative to start codonPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1073
Plasmid#53003PurposepCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsmCherry
GFP
UseSynthetic BiologyTagsompC (target mRNA) and sodB (target mRNA)ExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1394
Plasmid#53009PurposepLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
T7(st3) (133bps)
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1303
Plasmid#53008PurposepLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfpDepositorInsertssodB
ompC
UseSynthetic BiologyTagsGFP and mCherryExpressionBacterialMutationcontains -56 to +141 relative to start codon and …PromoterpLlacO-1 and pLtetO-1Available sinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
HL 3448 (=HL 3425 + pHL 1191)
Plasmid#30078DepositorInsertpLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 3375 (=HL 865 + pHL 1008)
Plasmid#30072DepositorInsertpLlacO-1:DsrA-mCherry, pLtetO-1:RBS (st7) gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
HL 3357 (=HL 716 + pHL 1191)
Plasmid#30068DepositorInsertpLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only