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Plasmid#211502PurposeAAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.DepositorTypeEmpty backboneUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pBS-PtH2B
Plasmid#133390PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT/Caggs-NRAS G12V-IRES-ASK1
Plasmid#221074PurposeExpresses NRAS G12V and ASK1 in mammalian cellsDepositorInsertsUseTagsExpressionMammalianMutationG12VPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3
Plasmid#61622PurposeFRET-based biosensor for monitoring cAMPDepositorInsertICUE3 (RAPGEF3 Human)
UseTagsECFP and cpVenus[L194]ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK349 (BSD-P2A-mAID-mCherry)
Plasmid#121183PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID-mCherry
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK346 (Hygro-P2A-mAID-mCherry)
Plasmid#121180PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID-mCherry
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK::tTA2-bGHpA
Plasmid#62448PurposeMXS_chaining vector with PGK::tTA2-bGHpADepositorInsertcassette containing the tetracycline transactivator with PGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS006
Plasmid#211506PurposeAAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequencesDepositorTypeEmpty backboneUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2NT
Plasmid#62257Purposeexpression of A2NT sgRNA from the arabinose-inducible promoterDepositorInsertA2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
P1-EYFP-pA (Construct 5)
Plasmid#55197PurposeSynthetic promoter responsive to gRNA1DepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-myc3-NEDD8
Plasmid#19943DepositorInsertNEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-LMP1-IRES-eGFP
Plasmid#36955DepositorInsertLMP1 (LMP-1 )
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pXint129
Plasmid#178208PurposeEncodes bacteriophage lamda excisionase and integrase to facilitate in vivo Gateway LR clonase reaction.DepositorUseTagsExpressionBacterialMutationPromoterlacIAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHRE
Plasmid#134909PurposeOverexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterdouble 35SAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hSAPS1-HA
Plasmid#165466PurposeHuman SAPS1 mammalian expression vector with C-terminal HA tag.DepositorInsertProtein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Luc-SIRT1 3'UTR
Plasmid#20379DepositorInsertSIRT1 (SIRT1 Human)
UseLuciferaseTagsExpressionMutation3'UTR of SIRT1PromoterAvailable sinceMay 1, 2009AvailabilityAcademic Institutions and Nonprofits only