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AiP14201: pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA (Alias: CN4201)

Plasmid

#214855

Purpose

Expression of FlpO in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-OmpA-NHSTIIT-GG

Plasmid

#215790

Purpose

Cloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-SufI-NHSTIIT-GG

Plasmid

#215791

Purpose

Cloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMV762-Peredox-mCherry

Plasmid

#90217

Purpose

Fluorescent reporter for NADH:NAD+ ratios in Mycobacteria.

Depositor

Ashwani Kumar

Article

Bhat et al Front Cell Infect Microbiol. 2016 Nov 8;

Insert

Codon Optimized Peredox-mCherry

Use

Shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

groEL

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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AiP12982 - pAAV-AiE0452h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2982)

Plasmid

#191709

Purpose

AI plasmid ALIAS: AiP12982. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12609 - pAAV-AiE0779m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2609)

Plasmid

#191705

Purpose

AI plasmid ALIAS: AiP12609. Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14132 - pAAV-AiE0779m_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4132)

Plasmid

#191735

Purpose

AI plasmid ALIAS: AiP14132. Expression of jGCaMP8m in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

jGCaMP8m

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14134 - pAAV-AiE0452h_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4134)

Plasmid

#191736

Purpose

AI plasmid ALIAS: AiP14134. Expression of jGCaMP8m in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

jGCaMP8m

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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GB4356

Plasmid

#187813

Purpose

Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and SpATF1-2.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Moth Pheromone pathway

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAGIG-WGA-mCherry

Plasmid

#224119

Purpose

To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.

Depositor

Pantelis Tsoulfas

Insert

WGA-mCherry

Use

AAV

Tags

WGA and mCherry

Expression

Mammalian

Mutation

All mutations related to the WGA in this construc…

Promoter

CAGIG

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GB3898

Plasmid

#187812

Purpose

Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and ScATF1.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Moth Pheromone pathway

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4360

Plasmid

#187814

Purpose

Module for dCasEV2.1 activated expression ATF1, Atr delta11 and HarFAR.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Moth Pheromone pathway

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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GB4361

Plasmid

#187815

Purpose

Module for dCasEV2.1 activated expression of ATF1-2, HarFAR and Atr delta11.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Moth Pheromone pathway

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4366

Plasmid

#187816

Purpose

Module for dCasEV2.1 activated expression of HarFAR, ATF1 and Atr delta11.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Moth Pheromone pathway

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4367

Plasmid

#187817

Purpose

Module for dCasEV2.1 activated expression for HarFAR, ATF1-2 and Atr delta11.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Moth Pheromone pathway

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)

Plasmid

#199777

Purpose

enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Has Service

AAV PHP.eB

Insert

iCre(R297T)

Use

AAV

Tags

Expression

Mutation

R297T for reduced recombinase efficiency.

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14696: pAAV-AiE0779m_6xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4696)

Plasmid

#214859

Purpose

Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1 MSNs), stronger than 3xCore version CN3044)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044)

Plasmid

#191706

Purpose

AI plasmid ALIAS: AiP13044. Optimized expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMVA

Plasmid

#121149

Purpose

This plamid was used for improving the production of DMAPP (IPP) in E.coli

Depositor

Minjuan Xu

Article

He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

Insert

MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237)

Plasmid

#191707

Purpose

AI plasmid ALIAS: AiP12237. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

none

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge