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pQCXIP-GFP-LmnB1

Plasmid

#164249

Purpose

retroviral plasmid for GFP-LmnB1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

LMNB1 (LMNB1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Article

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma13-GFP2

Plasmid

#140992

Purpose

Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma13-GFP2 (GNG13 Human)

Use

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBI-eGFP-Rab22

Plasmid

#200264

Purpose

Expression of eGFP-Rab22 (human) in mammalian cells

Depositor

Guangpu Li

Article

Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi

Insert

Rab22a (human cDNA) (RAB22A Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAdEasy-EGFP-Tubulin

Plasmid

#24411

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
GFP-Rab27B N133I

Plasmid

#89449

Purpose

Expression of GFP-tagged human Rab27B N133I in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B N133I (RAB27B Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

N133I

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Osh2-PH

Plasmid

#71373

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Use

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pBGSA C5aR1-EGFP

Plasmid

#62612

Purpose

Constitutive expression of C5aR1-EGFP in mammalian cells

Depositor

Heini Miettinen

Article

Suvorova et al Traffic. 2005 Feb;6(2):100-15.

Insert

C5aR1 (C5AR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
Influenza-FPV-HA-mEGFP

Plasmid

#127810

Purpose

Expression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localization

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+12)

Plasmid

#199161

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+12)

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-BRAT1

Plasmid

#161920

Purpose

Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

BRCA1-associated ATM activator 1 (BRAT1 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pg-EGFP-HIF-2alpha

Plasmid

#87207

Purpose

N-terminally EGFP-tagged human HIF-2a/EPAS

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-2a (EPAS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q868E

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJN105-Hygro-GFP

Plasmid

#41883

Depositor

Howard Steinman

Article

Bandyopadhyay et al Infect Immun. 2004 Aug;72(8):4541-51.

Insert

Ptac::GFP-mut-2

Use

Bacterial broad host range vector

Tags

Expression

Bacterial

Mutation

GFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…

Promoter

Ptac

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only
pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

Plasmid

#107576

Purpose

B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

GFP+

Use

Synthetic Biology

Tags

B. megaterium DSM319 xylA leader sequence (MTSSKI…

Expression

Bacterial

Mutation

F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

Promoter

pdh promoter Bacillus megaterium DSM319

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-MYO10-HMM

Plasmid

#87256

Purpose

Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413-GAL1-yEGFP1

Plasmid

#40235

Depositor

Michael Benton

Insert

yEGFP1

Use

Tags

HIS3MX6

Expression

Bacterial

Mutation

Promoter

GAL1

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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SBP-GFP-Tac

Plasmid

#162505

Purpose

Expresses GFP tagged Tac in a RUSH vector in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

Tac (IL2RA Human)

Use

Tags

GFP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pscALPS gfp Blasti

Plasmid

#115804

Purpose

Express gfp driven by SFFV promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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MRAP_pcDNA6.2/EmGFP-Bsd

Plasmid

#176937

Purpose

Mammalian expression vector encoding MRAP and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MRAP (MRAP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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