Addgene

We narrowed to 5,232 results for: arl

Showing: 4741 - 4760 of 5232 results

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pAAV-FLEX-NBL10

Plasmid

#69971

Purpose

Cre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusion

Depositor

Jeffrey Friedman

Article

Ekstrand et al Cell. 2014 May 22;157(5):1230-42. doi: 1

Insert

NBL10

Use

AAV and Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

Expression

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Promoter

Available since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2-Orai1

Plasmid

#73562

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1 (ORAI1 Synthetic, Human)

Use

Tags

G-GECO1.2

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SNX17 WT

Plasmid

#69811

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pdRBFC-B2-YN

Plasmid

#74721

Purpose

Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

Depositor

Aiming Wang

Article

Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

Insert

Influenza A virus NS1 protein, partial

Use

Double-stranded rna labeling

Tags

HA and YFP N-terminal half domain

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pdRBFC-B2-YC

Plasmid

#74722

Purpose

Express C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

Depositor

Aiming Wang

Article

Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

Insert

Influenza A virus NS1 protein, partial

Use

Double-stranded rna labeling

Tags

FLAG and YFP C-terminal half domain

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2-MACROD1

Plasmid

#172587

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2-MACROD1_d77

Plasmid

#172588

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

deleted amino acids 1-76

Promoter

CMV

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr3

Plasmid

#107998

Purpose

Expresses mouse Egr3 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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cycLuc_TEVS

Plasmid

#119207

Purpose

Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

cyclic luciferase with TEVs cleavage site

Use

Luciferase

Tags

6xHis and AU1 tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-sCre

Plasmid

#55635

Purpose

Expresses sCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

Cre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC841 - pAAV minP secNLuc

Plasmid

#82498

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

c-Fos minimal promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-165

Plasmid

#118577

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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G-GECO1-Orai1

Plasmid

#73561

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

NM_032790.3 (ORAI1 Synthetic, Human)

Use

Tags

G-GECO1

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-165

Plasmid

#118545

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-TRPM1 cDNA

Plasmid

#29782

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

TRPM1 (TRPM1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9

Plasmid

#162162

Purpose

Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

N/A

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf48

Plasmid

#12736

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf48

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pZDonor-Nm-Cas9-gRNA-hU6

Plasmid

#61366

Purpose

empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTag-RFP-C-h-Rab5a-c-Myc

Plasmid

#79802

Purpose

Express TagRFP labeled human Rab5a (GTPase located on early endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab5a (RAB5A Human)

Use

Tags

TagRFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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