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Plasmid#170662PurposeLentiviral expression of constitutively active STAT5A.DepositorInsertSTAT5A (STAT5A Human)
UseLentiviralTagsExpressionMutationH298R and S710FPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE1
Plasmid#132774PurposePrime editing in mammalian cellsDepositorInsertPE1
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXD054 KIKO CD19 CAR AAV vector
Plasmid#192157PurposeCD19 CAR AAV vectorDepositorInsertanti-CD19 CAR (CD19 Human)
UseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP_LEU2D
Plasmid#58352PurposeUsed for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiaeDepositorTypeEmpty backboneUseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 1
Plasmid#51760PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaz-RLuc8
Plasmid#140978PurposeEncodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaz-Rluc8 (GNAZ Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY002 (pFnCpf1_min)
Plasmid#69975PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes nd artificial promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-KRAB
Plasmid#50919PurposeConstitutive dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-mRuby2-Gephyrin.FingR-IL2RGTC
Plasmid#125696PurposeGlobal labeling of inhibitory synapses (red fluorescence)DepositorInsertmRuby2-Gephyrin.FingR-IL2RGTC
UseAAVTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-SADB19L
Plasmid#32632DepositorInsertSADB19L
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
EF1a-ABCD3-RFP-Puro
Plasmid#170168PurposeMammalian Expression of Ef1a-ABCD3-RFP-PuroDepositorInsertABCD3-RFP-Puro
UseTagsExpressionMammalianMutationn/aPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG461-FRB-redNFAST
Plasmid#160362PurposeExpresses FRB-redNFAST in mammalian cellsDepositorInsertFRB-redNFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
DsRed-rab11 DN
Plasmid#12680DepositorInsertRAB11A (Homo sapiens) (RAB11A Human)
UseTagsDsRed and HAExpressionMammalianMutationchanged serine 25 to asparaginePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+12)
Plasmid#199161Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+12)
UseTagsMGHHHHHHGGAExpressionMutationE16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphasS-RLuc8
Plasmid#140980PurposeEncodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphasS-RLuc8
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgCIC-2
Plasmid#74959PurposeCas9 + sgCIC-2 with blasticidin selectionDepositorInsertCIC (CIC Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-WT-IRES-mCherry
Plasmid#68856PurposeExpresses Human CDK8 Wild-TypeDepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY1
Plasmid#21760DepositorInsertECFP -EYFP linked by flexible one SGGSGG repeats
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
CD47-bio-His
Plasmid#53150PurposeExpresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD47 (CD47 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
mVenus-MAP4-C-10
Plasmid#56388PurposeLocalization: Microtubules, Excitation: 515, Emission: 528DepositorInsertMAP4
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Km
Plasmid#46813PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRTagsExpressionMutationD10APromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHRM5-Tango
Plasmid#66252PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertCHRM5 (CHRM5 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-Nanoluc
Plasmid#87075PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseLentiviralTagsNanoluc luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR4-24XPP7SL
Plasmid#31864DepositorInsert24xPP7 Stem Loop Cassette
UseTagsExpressionMutationPromoterAvailable sinceAug. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B
Plasmid#103002Purposenon-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-TurboID-NS
Plasmid#166566PurposeMultisite Gateway middle entry clone encoding TurboID. Parton lab clone JRPDepositorInsertTurboID
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIQmate
Plasmid#216240PurposeAll-in One IQ-Cumate transgene switch, empty vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-G418
Plasmid#160012PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Tol2
Plasmid#31831DepositorInsertTol2 Transposase
UseCloning vectorTagsExpressionMutationPromoterLacAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227APromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-6.20
Plasmid#41002DepositorInsertGSG-P2A-RLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceNov. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag FKHR
Plasmid#13507DepositorInsertFKHR (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon DREADD Gi-mCherry
Plasmid#177672PurposeExpresses DREADD Gi in Cre and Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
2X PRE TK luc
Plasmid#11350DepositorInsertPRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SNAP29
Plasmid#45915DepositorInsertsynaptosomal-associated protein, 29kDa (SNAP29 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLY094-EFS-CD22-Blast-WPRE
Plasmid#192195PurposeLenti-CD22-OE-BlastDepositorInsertLenti-CD22-OE-Blast (CD22 Synthetic)
UseLentiviral; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH10
Plasmid#188783PurposeCarries a NAT marker and C. albicans RBT5 promoter in a pGEM-T backboneDepositorInsertRBT5 promoter
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCMV-BE-zCas9
Plasmid#101739Purposeexpression of an optimized Cas9 for base-editing in zebrafishDepositorInsertrAPOBEC1-XTEN-zCas9-UGI-NLS
UseCRISPRTagsExpressionMammalianMutationD10APromoterCMVAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YAP1-V5 in pLX304
Plasmid#42555DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAD29_hIRG1_4-461_pvp008
Plasmid#124843PurposeExpresses human CAD (Irg1) in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
UseTagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-3 and 462-end.PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp6-His
Plasmid#11823DepositorInsertCaspase 6 (CASP6 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDF0234 pCMV - huDisCas7-11 dead mutant
Plasmid#172509PurposeEncodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cellsDepositorInsertpCMV - huDisCas7-11 dead mutant
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI819
Plasmid#216628PurposeCal-ID (V5) for mammalian expression and lentivirus productionDepositorArticleInsertCal-ID
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2
Plasmid#220243PurposeExpresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast
Plasmid#216733PurposeExpresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.DepositorArticleInsertCas9-D10A nickase and sgCTG
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10APromoterU6 and CAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/empty
Plasmid#105505PurposeEmpty backbone of MSCV-driven retroviral cDNA expressionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only