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Plasmid#86055PurposeA mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.DepositorInsertInterleukin-2 receptor subunit alpha (IL2RA Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
spNW50
Plasmid#173486PurposeExpresses spNW50 in Bl21DepositorInsertspNW50
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV:DsRed(FRT)GFP
Plasmid#31128DepositorInsertDsRed(FRT)GFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-hCas3
Plasmid#134920PurposeComponents for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.DepositorInsertCas3 with bpNLS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma2-GFP2
Plasmid#166774PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma2-GFP2 (GNG2 Human, Synthetic)
UseTagsExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 WT
Plasmid#121446Purposeexpression of huamn PD-L1 wildtype in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationwildtypePromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pLAT1_Cas9
Plasmid#104908PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418DepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.MaCPNS1
Plasmid#185136Purposenon-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBB540
Plasmid#27393DepositorInsertgrpE, clpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-Citroff1
Plasmid#134304PurposeMammalian expression of inverse-response citrate sensorDepositorInsertCitroff1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D2s-L-Venus
Plasmid#19966DepositorInsertD2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)
UseTagsFlag and monomeric VenusExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-sso7d-ligase
Plasmid#87745PurposeIPTG-inducible expression of sso7d-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized sso7d (Sulfolobus solfa…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-tagBFP
Plasmid#75160PurposeDerived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagstagBFPExpressionMammalianMutationPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJL1-eBFP2
Plasmid#102640PurposeIn vitro expression of eBFP2 from the T7 promoterDepositorInserteBFP2
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL-SaKKH-BE3
Plasmid#85170PurposeExpresses SaKKH-BE3 in mammalian cells [Edit]DepositorInsertSaKKH-BE3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet
Plasmid#138086PurposeEmpty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particlesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterminimal CMV promoter with heptamerized tet‐operat…Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-hERG1a
Plasmid#53052PurposeExpresses alpha subunit of hERG1a K channel in mammalian cellsDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-CUL5
Plasmid#19895DepositorInsertcullin 5 (CUL5 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-m
Plasmid#136294Purposeexpresses ABE8.8-m in mammalian cellsDepositorInsertABE8.8-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-U6-crRNA-empty
Plasmid#134921PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.DepositorInsertcrRNA for CRISPR-Cas3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
hWIP1 FLAG
Plasmid#28105DepositorInsertWIP1 (PPM1D Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD1
Plasmid#33108DepositorInsertTEAD1 (TEAD1 )
UseTagsGal4ExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMammalianMutationPromoterSynapsin and U6Available sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag1-NS3
Plasmid#17645DepositorInsertHCV NS3 (POLY Hepatitis C virus)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic cpVenus control
Plasmid#186788PurposeIdentical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligaseDepositorInsertcytoplasmic cpVenus control
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)
Plasmid#107941PurposeMammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutionsDepositorInserthuman codon optimized enAsCas12a (E174R/S542R/K548R)
UseTags3x HA and NLS (nucleoplasmin)ExpressionMammalianMutationE174R, S542R and K548RPromoterCAGAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE-KRAS
Plasmid#116755PurposeLentiviral expression of KRASDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)
Plasmid#133794PurposeU6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018DepositorInsertNmeCas9 and Nme2Cas9 sgRNA entry vector
UseTagsExpressionMammalianMutationsgRNA architecture from Amrani et al. Genome Biol…PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only