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Showing: 4721 - 4740 of 13536 results

  3. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid
    #197566
    Purpose
    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    N166A, V168G, W239C
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197566-plasmid-map-sequence-id-388228

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  6. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

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  10. Desmoplakin II acceptor only control (F40-based tension sensor)

    Plasmid
    #118719
    Purpose
    The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)(Y67G)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118719-plasmid-map-sequence-id-230445

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  11. FL

    Plasmid
    #120522
    Purpose
    Firefly luciferase reporter
    Depositor
    Petr Svoboda
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    120522-plasmid-map-sequence-id-233814

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  12. U6-MosIR

    Plasmid
    #120529
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Mos (Mos Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120529-plasmid-map-sequence-id-233976

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  13. H1-Mos-6U

    Plasmid
    #120531
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Mos (Mos Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    A>G (see comments)
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120531-plasmid-map-sequence-id-234010

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  14. CMV-MosIR

    Plasmid
    #120523
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Mos (Mos Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120523-plasmid-map-sequence-id-233706

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  15. pSV40_HA-mTarbp2

    Plasmid
    #120539
    Purpose
    protein expression
    Depositor
    Petr Svoboda
    Insert
    TARBP2 (Tarbp2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120539-plasmid-map-sequence-id-233983

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  16. U6-Mos-as

    Plasmid
    #120534
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Mos (Mos Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    A>G (see depositor comments)
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120534-plasmid-map-sequence-id-234013

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  17. U6-Mos-s

    Plasmid
    #120533
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Mos (Mos Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    A>G (see depositor comments)
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120533-plasmid-map-sequence-id-234047

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  18. CAG-EGFP-MosMos

    Plasmid
    #120515
    Purpose
    ssRNA expression
    Depositor
    Petr Svoboda
    Insert
    Mos (Mos Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120515-plasmid-map-sequence-id-233797

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  19. CMV-Mos-as

    Plasmid
    #120526
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Mos (Mos Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    120526-plasmid-map-sequence-id-233808

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  20. CMV-Mos-s

    Plasmid
    #120525
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Petr Svoboda
    Insert
    Mos (Mos Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    A>G (see comments)
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120525-plasmid-map-sequence-id-233801

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Showing: 4721 - 4740 of 13536 results