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Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV cleavable His10ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pET28_human-pro-IL-18_E192K_D193K
Plasmid#214322PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationE192K_D193KPromoterT7Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_I48N
Plasmid#214323PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationI48NPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_human-IL-18
Plasmid#214338PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_mouse-Pro-IL-18_humanized-triple-mutant
Plasmid#214339PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Mouse)
UseTagsHis-TEVExpressionBacterialMutationENGD29DDEN_N36Y_H41EPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_LES33AAA
Plasmid#214326PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationLES33AAAPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_DDEN29ENGD
Plasmid#214325PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationDDEN29ENGDPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_V47N_I48N
Plasmid#214324PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationV47N_I48NPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_human-pro-IL-18
Plasmid#214337PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_rat
Plasmid#214333PurposeProtein overexpression in bacteriaDepositorInsertIL18 (Il18 Rat)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-Halo-H2DBD
Plasmid#200206PurposeExpression of truncated HSF2 (DBD)DepositorInsertHA-Halo-H2DBD (Hsf2 Mouse)
UseTagsHalo; HAExpressionMammalianMutationHSF2 (1-366; 15 bp linker; 972-1017)PromoterEF_1alphaAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
H1DBD-Halo-HA
Plasmid#200203PurposeExpression of truncated HSF1 (DBD and HR)DepositorInsertH1DBD-Halo-HA (Hsf1 Mouse)
UseTagsHalo; HAExpressionMammalianMutationHSF1 (1-399)PromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
H1DBD-HR-Halo
Plasmid#200204PurposeExpression of truncated HSF1 (DBD and HR)DepositorInsertH1DBD-HR-Halo (Hsf1 Mouse)
UseTagsHaloExpressionMammalianMutationHSF1 (1-672)PromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229L-pET151
Plasmid#177879PurposeExpresses mouse Talin 1 F2F3-P229L domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-P229S-pET151
Plasmid#177871PurposeExpresses mouse Talin 1 F2-P229S domain in bacterial cellsDepositorInsertmouse Talin 1 F2 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2-P229L-pET151
Plasmid#177876PurposeExpresses mouse Talin 1 F2-P229L domain in bacterial cellsDepositorInsertmouse Talin 1 F2 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229S-pET151
Plasmid#177878PurposeExpresses mouse Talin 1 F2F3-P229S domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF101.1gw3
Plasmid#172181PurposeA binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorInsertattR3-CmR-ccdB-attR4
UseTagsExpressionPlantMutationPromoterlacUV5 promoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only