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Plasmid#179717PurposeLentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFPDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt7
Plasmid#213630PurposeBacterial expression of SUMO-tagged mouse FL-syt7DepositorInsertFL-syt7 (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PTK6-YF
Plasmid#210715PurposeConstruct for stable expression of constitutively active human PTK6 in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (PTK6 Human)
UseTagsExpressionMammalianMutationTyrosine 447 changed to PhenylalaninePromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
XPO6-2Strep
Plasmid#210356PurposeExpresses XPO6 fused with 2Strep in mammalian cellsDepositorInsertXPO6 (XPO6 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM2-2Strep
Plasmid#210374PurposeExpresses TRAM2 fused with 2Strep in mammalian cellsDepositorInsertTRAM2 (TRAM2 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM1-2Strep
Plasmid#210352PurposeExpresses TRAM1 fused with 2Strep in mammalian cellsDepositorInsertTRAM1 (TRAM1 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
SGTA-2Strep
Plasmid#210353PurposeExpresses SGTA fused with 2Strep in mammalian cellsDepositorInsertSGTA (SGTA Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR54
Plasmid#203330Purposeepisomal bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPRL1_1-169
Plasmid#209792PurposeBacterial expression of human PRL-1 without farnesylation motifDepositorInsertPRL-1 (PTP4A1 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPRL1_1-169_C104D
Plasmid#209793PurposeBacterial expression of human PRL-1 C104D mutant without farnesylation motifDepositorInsertPRL-1 (PTP4A1 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 only; C101DPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPRL3_1-169_C104D
Plasmid#209797PurposeBacterial expression of human PRL-3 C104D mutant without farnesylation motifDepositorInsertPRL-3 (PTP4A3 Human)
UseTags6xHisExpressionBacterialMutationaa 1-169 only; C101DPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB398
Plasmid#203634PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertFB367+FB395
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB399
Plasmid#203635PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertFB367+FB396
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR24
Plasmid#202771Purposeepisomal bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coNAT
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWR16
Plasmid#202711Purposeintegrating bioluminescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB400
Plasmid#203636PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertFB367+FB397
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-GPI1
Plasmid#202124PurposeLevel 0 plasmid containing the promoter from gene 23924 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertGPI1 promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-fcpF
Plasmid#202119PurposeLevel 0 plasmid containing the promoter from gene 51230 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertfcpF promoter
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits