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Showing: 4701 - 4720 of 27422 results

  1. p4B5-EGFP

    Plasmid
    #140324
    Purpose
    Detection of Dengue virus infection in live cells
    Depositor
    Alan Rothman
    Article
    Medin et al J Virol Methods. 2015 Jan;211:55-62. doi
    Insert
    DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    2 conservative n.t. differences near 5' end …
    Promoter
    CMV
    Available since
    May 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    140324-plasmid-map-sequence-id-296784

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  7. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    et al Nat Commun 15
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  8. pre_IV_CS10_1545_v1

    Plasmid
    #126861
    Purpose
    4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation
    Depositor
    Hendrik Dietz
    Article
    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn
    Insert
    IV_CS10_1545_v1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    126861-plasmid-map-sequence-id-250149

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  10. pNICKclos1.0

    Plasmid
    #73639
    Purpose
    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    Cas9 nickase
    sGRNA to pyrE
    Use
    E.coli-clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A
    Promoter
    j23119 and ptb
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73639-plasmid-map-sequence-id-148603

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  18. CAG-Cremyc-2A-GFP

    Plasmid
    #116879
    Purpose
    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.
    Depositor
    Qiang Lu
    Article
    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:
    Insert
    Cremyc-2A-GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    116879-plasmid-map-sequence-id-243398

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Showing: 4701 - 4720 of 27422 results