Addgene

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pGD253

Plasmid

#132374

Purpose

contains the sequence of mRuby2 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mRuby2

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133712

Plasmid

#152678

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;TapTag

Expression

Mammalian

Mutation

H. sapiens recode 1

Promoter

CMV

Available since

June 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LCMV:ECFP(FRT)EYFP

Plasmid

#31307

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(FRT)EYFP

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-mPar6a ΔPro136

Plasmid

#26666

Depositor

Senthil Muthuswamy

Article

Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

Insert

Flag Par6a

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pTCS-mcs

Plasmid

#31288

Depositor

Dirk Schnappinger

Article

Venugopal et al Cell Host Microbe. 2011 Jan 20. 9(1):21-

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
Flag-mpar6a M235W

Plasmid

#26668

Depositor

Senthil Muthuswamy

Article

Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

Insert

Par6a

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pGBW-m4252811

Plasmid

#153563

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pKER-mCardinal

Plasmid

#85976

Purpose

Expresses mCardinal from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mCardinal

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133578

Plasmid

#152329

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-FLEX-tTA

Plasmid

#149363

Purpose

AAV-mediated and Cre-dependent expression of tTA under the CAG promoter.

Depositor

Takeshi Imai

Article

Leiwe et al bioRxiv 2022.10.20.512984

Insert

tTA2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4252852

Plasmid

#153644

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
TALE5

Plasmid

#35420

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE5

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEmerald-SpHis5

Plasmid

#44851

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Emerald with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEmerald

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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TALE10

Plasmid

#35425

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE10

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
TALE12

Plasmid

#35427

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE12

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
TALE11

Plasmid

#35426

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE11

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
TALE6

Plasmid

#35421

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE6

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yoMaxGFP-Kan

Plasmid

#44907

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCARCre

Plasmid

#31309

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

cardiac actin / Cre

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
piHGpd/P82

Plasmid

#1325

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

GPD promoter-HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Wild type gene

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only