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Plasmid#107694PurposeOsActin Promoter-LwaCas13a-HSP TerminatorDepositorInsertCas13a
UseTagsExpressionPlantMutationPromoterOsActinAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-S-LexA::p65
Plasmid#133564PurposeSwap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet
Plasmid#125134PurposeNatively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-UBCp-hMGT-Ef1a-puro
Plasmid#172396PurposeLentiviral expression vector for human direct cardiac reprogrammingDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF184
Plasmid#89482PurposeExpress asparaginyl endopeptidase 1 from O. affinis (OaAEP1)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
UseTagsSmt3ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-HA-UFL1
Plasmid#139860PurposeLentiviral expression of HA-tagged human UFL1DepositorInsertUFL1 (UFL1 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-PH-BTKx2
Plasmid#183654PurposePtdIns(3,4,5)P3 biosensorDepositorInsertBTK (BTK Human, Frog)
UseTagsX. laevis map2k1.L(32-44)-mCherryExpressionMammalianMutationAmino acids 2-174 and 2-170PromoterCMVAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mWasabi-C1
Plasmid#54743PurposeLocalization: C1 Cloning Vector, Excitation: 493, Emission: 509DepositorTypeEmpty backboneUseTagsmWasabiExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF0235
Plasmid#142488PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYCN (MYCN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKTol2C-EGFP
Plasmid#85598PurposeTol2 transposon with EGFP driven by minimal-CAGs promoter.DepositorInsertTol2 transposon with EGFP
UseTagsExpressionMammalianMutationPromotermini-CAGAvailable sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pLenti 4.1 Ex miR200b-200a-429
Plasmid#35533Purpose3rd generation lentiviral vectorDepositorUseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-NR1a
Plasmid#17928DepositorInsertN-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorInsertOTULIN (OTULIN Human)
UseTagsHis6-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo
Plasmid#139449PurposeLentiviral vector with gRNA scaffold and neomycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: neoR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJA42
Plasmid#59930PurposegRNA for cleavage at rol-6(su1006) locus in C elegansDepositorInsertrol-6(su1006) gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMVdel-dimericTomato-WASp(CRIB)
Plasmid#191450PurposeVisualizing endogenous & active Cdc42DepositorInsertWASp
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only