Addgene

We narrowed to 15,717 results for: puromycin

Showing: 4641 - 4660 of 15717 results

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WEE1 F10.4 gRNA

Plasmid

#90941

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F10.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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WEE1 F9.4 gRNA

Plasmid

#90940

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F9.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLib6.4B

Plasmid

#176665

Purpose

Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_TFRC_C (with mCherry)

Plasmid

#72627

Purpose

Expresses two gRNAs targeting the TFRC promoter

Depositor

Roderic Guigo, Rory Johnson

Article

Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

Insert

gRNAs toward TFRC

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 (gRNA1) and H1 (gRNA2)

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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FOXM1 C11.2 gRNA

Plasmid

#90690

Purpose

3rd generation lentiviral gRNA plasmid targeting human FOXM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FOXM1 (Guide Designation C11.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-ERas-IP

Plasmid

#13826

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR-v1-sgMPC2_7

Plasmid

#163457

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 7 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMPC2_9

Plasmid

#163458

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 9 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Rad23b UbL

Plasmid

#184703

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbL

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Rad23b UbL

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pIRESpuro-Flag-Pol b(K72A)

Plasmid

#23258

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan 12. ():.

Insert

DNA polymerase beta (K72A) (POLB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K72A

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pmiReport SHIP1 3' UTR

Plasmid

#26531

Depositor

David Baltimore

Article

O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

Insert

SHIP1 3'UTR (Inpp5d Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
KIF11 F5.3 gRNA

Plasmid

#90716

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF11

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF11 (Guide Designation F5.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagBcl10K31RK63R

Plasmid

#18719

Depositor

Jon Ashwell

Article

Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

B cell lymphoma 10 (BCL10 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Lysine 31 to arginine and Lysine 63 to arginine.

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pSIREN-RetroQ-CDK6-shRNA

Plasmid

#25789

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK6 (CDK6 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pLKO1-puro-U6-sgRNA-TGM2

Plasmid

#69239

Purpose

U6 driven SpCas9 sgRNA expression for TGM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TGM2 sgRNA (TGM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shABCB7_2

Plasmid

#102969

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_2 (ABCB7 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shABCB7_1

Plasmid

#102968

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_1 (ABCB7 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.MYB

Plasmid

#184660

Purpose

Retroviral expression of mouse RAP1 delta MYB

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta MYB

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.COIL

Plasmid

#184659

Purpose

Retroviral expression of mouse RAP1 delta COIL

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

pLPC

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta COIL

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.RAP1.delta.BRCT

Plasmid

#184658

Purpose

Retroviral expression of mouse Rap1 delta BRCT

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

RAP1

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

rap1 delta BRCT

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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