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Plasmid#167311PurposeTemplate pDNA to prepare a split CaVT component mRNADepositorInsertHaloTag-VPg(FCV)
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-TOMM20-mEGFP
Plasmid#115923Purposedonor to insert EGFP at the TOMM20 locus in human cellsDepositorInsertTOMM20-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_nat
Plasmid#184911PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLC-242
Plasmid#73189PurposeTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertchloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
UseTagsExpressionBacterialMutationPromoterchloramphenicol promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_mic
Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgKEAP1-2
Plasmid#74986PurposesgKEAP1-2, puromycin selectionDepositorInsertKEAP1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)
Plasmid#70191PurposeDerived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.DepositorInsertClpX (clpX )
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-SEC61B-mEGFP
Plasmid#115921Purposedonor to insert mEGFP at the SEC61B locus in human cellsDepositorInsertSEC61B-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-GAPDH-IRES-dTomato
Plasmid#115919Purposedonor to insert IRES-dTomato at the GAPDH locus in human cellsDepositorInsertIRES-dTomato
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTargeting_Mettl3C_FKBP-V
Plasmid#165421PurposeTemplate for knockin FBKP-V into Mettl3 C-terminusDepositorInsertMettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)
UseMouse TargetingTagsFKBP-VExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-30-picky_sticky
Plasmid#138983PurposeIVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38DepositorInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-33-MC_hammer
Plasmid#138990PurposeIVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-32-riot_punisher
Plasmid#138988PurposeIVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-32-riot_punisher
Plasmid#138987PurposeIVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38DepositorInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-31-murderous_crow
Plasmid#138986PurposeIVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRA-31-murderous_crow
Plasmid#138985PurposeIVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38DepositorInsertTCRA (Tcra Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-MC38-TCRB-30-picky_sticky
Plasmid#138984PurposeIVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38DepositorInsertTCRB (Tcrb Mouse)
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBCMV-MS2CP-eDHFR
Plasmid#167309PurposeTemplate pDNA to prepare a split CaVT component mRNADepositorInsertMS2CP-eDHFR
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only