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pLJM1 Flag GFP 4E-BP1 Omp25

Plasmid

#112760

Purpose

express GFP-4EBP1-Omp25

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-GFP-4EBP1-Omp25 (EIF4EBP1 Human)

Use

Lentiviral

Tags

Flag eGFP and Omp25

Expression

Mutation

Promoter

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CAMKV

Plasmid

#20443

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CAMKV (CAMKV Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pX330-Flag-wtSpCas9 (without sgRNA)

Plasmid

#92353

Purpose

Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-wild-type SpCas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(WT)--IRES-hyg

Plasmid

#128667

Purpose

mammalian expression of Flag-Polß(WT) protein with hygromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q85

Plasmid

#111736

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q85 (polyQ repeat)

Promoter

CMV and P10

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-N-Myc-Flag-BirA-hPOT1

Plasmid

#166409

Purpose

express BirA-hPOT1 in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Tags

BirA, flag, and myc

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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p6721 MSCV-IP N FlagHA 16E6 8S9A10T

Plasmid

#44153

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

E6 8S9A10T mutant

Promoter

MSCV LTR

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
p6722 MSCV-IP N FlagHA 16E6 I128T

Plasmid

#44154

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

HPV16 E6 I128T mutant

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 EPSM A208S

Plasmid

#27112

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T179V S204A S208A S213A; A208S

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta Myb

Plasmid

#13253

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Myb (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-131 + 184-399 and the point mu…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag VRK2

Plasmid

#20663

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

VRK2 (VRK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag TIE1

Plasmid

#20651

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TIE1 (TIE1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos DD T325A T331A

Plasmid

#8973

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D, T325A, T331A

Promoter

Available since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag OXSR1

Plasmid

#20553

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

OXSR1 (OXSR1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag ITPKB

Plasmid

#20550

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ITPKB (ITPKB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
V179 pLP Triple-Flag - Acceptor

Plasmid

#11706

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pGSTag Flag Jnk3a1(2nd methione)

Plasmid

#15746

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1-2nd M (MAPK10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV-RNF8-N-terminus(1-111)

Plasmid

#64674

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Only N terminus amino acids 1-111

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat 1-460

Plasmid

#33324

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

truncated at 460; S3V, G96R, and P108L also prese…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only