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Plasmid#89779Purposeyeast expression of mouse PKC Alpha with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Alpha (Prkca Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + codon optimized Brn3a
Plasmid#86829Purposemammalian expression of the transcription factor Brn3aDepositorInsertBrn3a (POU4F1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-Gateway
Plasmid#114011Purpose3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNADepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b – E. coli RecE residues 564-868
Plasmid#104534PurposeExpresses E. coli RecE residues 564-868DepositorInsertRecE residues 564-866 (C-terminal nuclease domain)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BirA-eCPX-AP(K10)
Plasmid#121908PurposeExpresses BirA and eCPX-AP(K10)DepositorInsertsUseTags6xHisExpressionBacterialMutationchanged lysine 10 in the AP to alaninePromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_SbMVs
Plasmid#119209PurposeCircularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with SbMVp cleavage site
UseLuciferaseTagsAU1 tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM108
Plasmid#133665PurposeIntegration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM12
Plasmid#133638PurposeIntegration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorInsertpCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PROM_MpUBE2
Plasmid#136109PurposePROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.DepositorInsertPROM_MpUBE2 Mapoly0106s0019
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
5UTR_MpUBE2
Plasmid#136110Purpose5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.DepositorInsert5UTR_MpUBE2 Mapoly0106s0019
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-LAP1_2-mCherry
Plasmid#159526PurposeExpresses mCherry from LAP1_2 promoterDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterLAP1_2Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-4FLPo
Plasmid#122050PurposeGenome plasmid for deletion-mutant rabies virus encoding Flpo recombinaseDepositorInsertFLPo
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNMHCII-C1C2-GFP
Plasmid#46044DepositorInsertnonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pAPcrRNA_Eco
Plasmid#106276PurposeE.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEcoCascade-IL1RNcrRNA
Plasmid#126481PurposeEncodes E.coli CRISPR Type I-E crRNA targeting IL1RNDepositorInsertEco IL1RN cr26
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/hTID1
Plasmid#13745DepositorInserthTid1 siRNA (DNAJA3 Human)
UseRNAiTagsExpressionMutationInverted repeat of 21 bp with 6 nuc. spacer.PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
yTREX
Plasmid#177306Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5DepositorTypeEmpty backboneUseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA027
Plasmid#216007PurposeFragmid fragment: (Cas protein) nearly PAM-lessDepositorInsertCas9-SpRY_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only