-
Plasmid#53604PurposeReporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D_dN
Plasmid#176465Purposeexpress VPS13D lacking N-terminal 117 aa in mammalian cellsDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK103
Plasmid#110582PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertLac promoter with lac operator and RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
R4pGWB401+Citrine
Plasmid#128432PurposeModified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.DepositorTypeEmpty backboneUseGateway destination vectorTagsCitrine, yellow fluorescence proteinExpressionPlantMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BpiI restriction sites were r…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVRc15_524
Plasmid#49736DepositorInsertAS15_524
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJM149
Plasmid#134168PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC E118A R129A, E. coli codon optimized, CRISPR…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM147
Plasmid#134167PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
JBEI-6833
Plasmid#84983PurposeExpression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.DepositorInsertNudB-PMD
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDN-NG1Tt
Plasmid#44523DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationPromoterPGal1 (wt)Available sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVRa41_491
Plasmid#49702DepositorInsertECF41_491
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa32_1122
Plasmid#49689DepositorInsertECF32_1122
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTX208
Plasmid#89268PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02DepositorInsertOsYSA-gRNA01 and OsYSA-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterMaize Ubiquitin1 promoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-N-Myc-Flag-BirA-hPOT1
Plasmid#166409Purposeexpress BirA-hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsBirA, flag, and mycExpressionMammalianMutationPromotercmvAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pRK-flag-USP19 494-1290
Plasmid#78580PurposeExpression flag-USP19 494-1290 in mammalian cellsDepositorInsertflag-USP19 494-1290 (USP19 Human)
UseTagsflagExpressionMammalianMutationdeleted amino acid 1-493 and mutated 1291 residue…PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRb18_up1700
Plasmid#49712DepositorInsertsfgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPecf18_up1700Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGW-dpse-GAL4-DBD
Plasmid#125153PurposeGateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CPDepositorInsertD. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
UseGateway fly expression vectorTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only