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pCMV-ABEmax(TadA E59A, TadA*D108F)

Plasmid

#125654

Purpose

pCMV-ABEmax(TadA E59A, TadA*D108F) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* D108F)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*V106M)

Plasmid

#125650

Purpose

pCMV-ABEmax(TadA E59A, TadA*V106M) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* V106M)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*V106F)

Plasmid

#125652

Purpose

pCMV-ABEmax(TadA E59A, TadA*V106F) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* V106F)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTR

Plasmid

#118615

Purpose

expression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoter

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7-E6 3'UTR (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

tetON CMV

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) TSPAN9

Plasmid

#107502

Purpose

Expresses TSPAN9, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

TSPAN9 (TSPAN9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 L128C/H113E

Plasmid

#111778

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L128C/H113E

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 T336A/F337A

Plasmid

#111787

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T336A/F337A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 Q340A/D341A

Plasmid

#111788

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Q340A/D341A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP

Plasmid

#99730

Purpose

overexpression of Tmeff2 and mCherry

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Use

Tags

2xMyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-Tmeff2-3xFlag-EF1-nlsCopGFP

Plasmid

#99729

Purpose

overexpression of Tmeff2 and CopGFP

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Rat NCKX2-AS-long-FlagTag (pRc/CMV)

Plasmid

#75195

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Tsoi et al J Biol Chem. 1998 Feb 13;273(7):4155-62.

Insert

SLC24A2

Use

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-mOC-STAMP (N162D)-GFP

Plasmid

#73580

Purpose

Lentivirus expression of mOC-STAMP with N162D mutation

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mutation

changed asparagin (N) 162 to Aspartic Acid (D)

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29-Mid/pCMV-14-FLAG (L175-D390)

Plasmid

#73589

Purpose

Express middle part (L175-D390) of human TRAFD1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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mOC-STAMP (N162D)_3xFlag-Myc-CMV26

Plasmid

#73578

Purpose

Express mouse OC-STAMP ((N162D) in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Tags

3x Flag and c-Myc tag

Expression

Mammalian

Mutation

changed asparagin (N) 162 to Aspartic Acid (D)

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 S592A

Plasmid

#61219

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Changed Serine 592 to Alanine

Promoter

CMV/TO

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pLA CMV N-Flag RALB V23 R47

Plasmid

#50983

Purpose

Expresses RALB-V23/R47 in mammalian cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

G23V, K47R

Promoter

CMV

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pLA CMV N-Flag RALB V23 R160

Plasmid

#50982

Purpose

Expresses RALB-V23/R160 in mammalian cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

G23V K160R

Promoter

CMV

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

Plasmid

#140003

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

Promoter

CMV and T7

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

Plasmid

#153005

Purpose

Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (C) (G79)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

Plasmid

#221233

Purpose

Express sgRNA targeting HEK3 loci with nCas9(D10A)

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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