Addgene

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pMSCVpuro-Gcn5(D608A)

Plasmid

#63707

Purpose

retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

D608A mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

Plasmid

#114089

Purpose

CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized FnCas12a

Use

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.S72A

Plasmid

#106200

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

GltI: S72A

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD_His B_QuasAr2

Plasmid

#64134

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD_His B_QuasAr1

Plasmid

#64135

Purpose

Fluorescent reporter for membrane voltage with improved brightness

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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FCK-Chronos-GFP

Plasmid

#80902

Purpose

Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184V

Plasmid

#106187

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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Zebrafish ArcLight Q175

Plasmid

#53616

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Zebrafish ArcLight-Q175 (tpte Aequorea victoria, Synthetic, Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Dr-VSD contains R153Q mutation and an amino acid …

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO_HOXB13_#2

Plasmid

#70094

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLKO_HOXB13_#1

Plasmid

#70093

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAAV.GFAP.SF-iGluSnFR.A184V

Plasmid

#106193

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgCRTC2-1

Plasmid

#138700

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgCRTC2-2

Plasmid

#138701

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mGSDMD1-251

Plasmid

#111565

Purpose

Express ELANE-cleaved mouse GSDMD in mammalian cells

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only
pEpicVector3mC_Puro-T2A-mCherry-VSVg-StrepTagII-HA

Plasmid

#162100

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-StrepTagII-HA

Use

Lentiviral

Tags

VSVg-StrepTagII-HA

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

Plasmid

#26797

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)

Use

Tags

Expression

Mammalian

Mutation

Human 5HT4B receptor with D100A mutation, generat…

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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5XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111545

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChrimson_tdtomato_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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OA-1136J

Plasmid

#153023

Purpose

Express His-MBP-TEV-CasRx recombinant protein

Depositor

Omar Akbari

Article

Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

Insert

CasRx

Use

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST

Plasmid

#182547

Purpose

Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificity

Depositor

Robert de Vries

Article

Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

Insert

H5_Vietnam_1203_04_Y161A

Use

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Y161A mutant that confers exclusive NeuAc to NeuG…

Promoter

CMV

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-plk1-AKAP

Plasmid

#132967

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1-AKAP (PLK1, AKAP9 Human)

Use

Tags

Expression

Mammalian

Mutation

C terminus of full-length Plk1 fused to the peric…

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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