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Plasmid#198511PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF0663
Plasmid#141654PurposeLentiviral vector for overexpressing the MNT transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMNT (MNT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadDE
Plasmid#193837PurposeExpress TadDE (with SpCas9) in mammalian cellsDepositorInsertTadA-DualEditor-Cas9D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Hygro
Plasmid#139462PurposeLentiviral vector with gRNA scaffold and hygromycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: hygroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF1231
Plasmid#144280PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertAFF3 (AFF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-RUNX3
Plasmid#139812Purposedoxycycline-inducible expression of Human RUNX3 in mammalian cellsDepositorInsertRUNX3 (RUNX3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-P2A-HSA
Plasmid#121936PurposeCRISPR-Sirius plasmidDepositorInsertdCas9-P2A-HSA
UseCRISPR and LentiviralTagsHSAExpressionMammalianMutationPromoterCMV-TOAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-VP64 human MyoD-T2A-dsRedExpress2
Plasmid#60629PurposeExpresses VP64 human MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP64 human MyoD (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/intron/ARE
Plasmid#62858Purposesplicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationinsertion of chimeric intron at nueotide poclsiti…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2
Plasmid#136579Purposemammalian expression of mRFP1-tagged human NRF2DepositorInsertNRF2 (NFE2L2 Human)
UseRetroviralTagsN-mRFP1-FLAGExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiLAMP5e3_jGCaMP8m
Plasmid#213918PurposeAAV construct expressing jGCaMP8m driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_jGCaMP8mDepositorInsertjGCaMP8m
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- SIV-MAC251 vpx
Plasmid#115810PurposeEncodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance proteinDepositorInsertSIVMAC251 Vpx
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR1a
Plasmid#17926DepositorInsertglutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4- miR30-L1221
Plasmid#115846PurposeControl shRNA KD vectorDepositorInsertcontrol shRNA
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL271
Plasmid#70254PurposesacB plasmid with polylinker; chloramphenicol/erythromycin resistance: use as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eNpHR 3.0-EYFP
Plasmid#26972PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibitionDepositorHas ServiceAAV5InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP720-N1
Plasmid#136560PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720DepositorInsertmiRFP720
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
IMS-APEX2
Plasmid#79058PurposeExpresses APEX in the mitochondrial intermembrane space.DepositorInsertAPEX
UseTagsV5ExpressionBacterial and MammalianMutationPromoterT7 promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0728
Plasmid#143434PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMLXIP (MLXIP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only