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Plasmid#171509Purposedeletion of a genomic locus in Prdm14 geneDepositorInsertPrdm14 (Prdm14 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A-Prdm14-L3-#2
Plasmid#171510Purposedeletion of a genomic locus in Prdm14 geneDepositorInsertPrdm14 (Prdm14 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A-Prdm14-L4-#1
Plasmid#171511Purposedeletion of a genomic locus in Prdm14 geneDepositorInsertPrdm14 (Prdm14 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A-Prdm14-L4-#2
Plasmid#171512Purposedeletion of a genomic locus in Prdm14 geneDepositorInsertPrdm14 (Prdm14 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Immune checkpoint (library 2) Backbone
Plasmid#192363PurposePlasmid vector for in vivo immune checkpoint screening library (for checkpoint library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
T cell activation (library 1) Backbone
Plasmid#192364PurposePlasmid vector for in vitro Jurkat activation screening (for immune library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC mTagBFP2-Synapsin1a
Plasmid#191567PurposeExpresses an shRNA and mTagBFP2-synapsin1aDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matMYC-C312S
Plasmid#115256PurposeFor mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with an internal MYC tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationC312SPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matMYC-ex2trunc
Plasmid#115257PurposeFor mammalian expression of a C-terminal truncated human NODAL open reading frame with an internal MYC tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationC-terminal truncationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matDYK-C312S
Plasmid#115259PurposeFor mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationC312SPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-T7TS-C312S
Plasmid#115263PurposeFor in vitro transcription of human NODAL open reading frame (with a mutated Cysteine residue) RNADepositorInsertNODAL (NODAL Human)
UseTagsExpressionBacterialMutationC312SPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-T7TS-N72A-N199A
Plasmid#115264PurposeFor in vitro transcription of human NODAL open reading frame (with two mutated N-glycosylation sites) RNADepositorInsertNODAL (NODAL Human)
UseTagsExpressionBacterialMutationN72A N199APromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF4E9D
Plasmid#185844PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>LacI>TUP1>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9C3
Plasmid#185843PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>RevTetR_r1.7>SIN3C>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D91
Plasmid#185845PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>SIN3C>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFZifPZ432
Plasmid#185850PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>ZifPZ43>MED15>PAFB1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D93
Plasmid#185847PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>MIG1C>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF9D92
Plasmid#185846PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>TetR>TUP1>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-CylK-Strep
Plasmid#185962PurposeExpresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparationDepositorInsertCylK
UseTagsHis6-SumoExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only