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Plasmid#133662PurposeIntegration of MNN9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorInsertp1k-MNN9-mTagBFP2 (MNN9 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-VChR1-EYFP-WPRE
Plasmid#20954Purpose3rd gen lentiviral expression of VChR1 fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorInsertvolvox channelrhodopsin-1
UseLentiviralTagsYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
ZJOM1
Plasmid#133632PurposeIntegration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.DepositorInsertp1k-GFP-SED5 (SED5 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EDD
Plasmid#37190DepositorInsertEDD (UBR5 Human)
UseTags6x His Tag and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAS9i
Plasmid#141249PurposeGalactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterGAL1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAS9c
Plasmid#141250PurposeConstitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorInsertp1k-ANP1-GFP (ANP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorInsertSEC7-2GFP (SEC7 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE165
Plasmid#118513Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE
Plasmid#20944Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationH134RPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFLEX-m-cyclin D1 -T286A mutant
Plasmid#86792Purposemammalian expression of cyclin D1 T286A mutantDepositorInsertcyclin D1 (Ccnd1 Mouse)
UseTagsFLAGExpressionMammalianMutationT286APromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVie-IRES-BlastR alt MCS
Plasmid#120862Purpose3rd generation lentiviral expression under a CMVie promoter with Blasticidin selectionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 (RNAi-res)
Plasmid#136645PurposeExpression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1 (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTCPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-miRFP670nano-miRFP670
Plasmid#216131PurposeLentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.DepositorInsertEF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)
Plasmid#136646PurposeExpression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1R312A (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-4RFP
Plasmid#52489PurposeExpresses TagRFP-TDepositorInsertTagRFP-T
UseRabies virusTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Gal4-GBP2-IRES-GBP7-p65
Plasmid#50020PurposeMulti-purpose expression of a GFP-dependent transcription factorDepositorInsertGal4DBD-GBP2-IRES-NLS-GBP7-p65AD
UseMulti-purpose expressionTagsExpressionMutationPromoterCMV-immediate early promoter and SP6 promoterAvailable sinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-Rab5-Blasticidin
Plasmid#120715PurposeExpresses early endosome-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertRab5a
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationAmino acids 2-215PromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM103
Plasmid#133664PurposeIntegration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_PPVs
Plasmid#119208PurposeCircularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with PPVp cleavage site
UseLuciferaseTagsAU1 tagExpressionMutationPromoterCMVAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only