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pAAV-G88P3-HA-hM3Dq

Plasmid

#213972

Purpose

AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

G88P3

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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MAC-CTSB

Plasmid

#172413

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Cathepsin B (CTSB Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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DRH322: AAV-CaMKIIa-QuasAr2-mO2

Plasmid

#51696

Purpose

AAV production of QuasAr2 fluorescent reporter

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

AAV

Tags

mOrange2

Expression

Mutation

Promoter

CaMKIIa

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-EphB4-SV40-GFP (B4G)

Plasmid

#65444

Purpose

Expression of human EPHB4 and GFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

EPHB4 (EPHB4 Human)

Use

Lentiviral

Tags

SV40-EGFP

Expression

Mammalian

Mutation

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-rc[Chronos-GFP]

Plasmid

#59056

Purpose

Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pFL His-p97

Plasmid

#113510

Purpose

Expression of His-p97 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97 (VCP Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184V

Plasmid

#106199

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf1(WT)-mCherry

Plasmid

#79419

Purpose

Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf1 (ARF1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3T911A

Plasmid

#120254

Purpose

Expression of ATP3-6 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

T911 changed to A; Isoleucine 304 changed to Aspa…

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF2205

Plasmid

#144004

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

POU2F3 (POU2F3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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13XLexAop2 IVS ChrimsonR mCherry_tr

Plasmid

#111548

Purpose

Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR_mCherry_trafficked

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

Plasmid

#114068

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for LbCas12a crRNAs

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

EF1a-short; U6

Available since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

Plasmid

#114067

Purpose

T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for AsCas12a crRNAs

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(WT)-mCherry

Plasmid

#79421

Purpose

Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

Plasmid

#63139

Purpose

Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

Depositor

Titia Sixma

Article

Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

Inserts

Ring1b (Ring1 Mouse)
Bmi1 (Bmi1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 1-109 and Residues 1-159

Promoter

tac

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

Plasmid

#114087

Purpose

U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

entry vector for FnCas12a crRNAs

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEN25-Ub Hs

Plasmid

#83232

Purpose

pHis-par2-Ub Hs

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Ubiqutin (UBC Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS009: pLX304-QuasAr2

Plasmid

#62985

Purpose

Lentivirus production of QuasAr2 fluorescent reporter

Depositor

Adam Cohen

Article

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Lentiviral; Gateway destination vector

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf3(WT)-mCherry

Plasmid

#79420

Purpose

Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf3 (ARF3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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