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Plasmid#69769PurposeThisbe (FGF8-like-1) Ligand in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertThisbe (FGF8like-1)
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationEncodes amino acids 1-739 of Thisbe (NP_610701.2)…Promoterhsp70 promoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
BII-ChiNW-ASCL2
Plasmid#133384PurposePiggybac vector for constitutive expressionDepositorInsertASCL2 (ASCL2 Human)
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
VQR variant
Plasmid#138560PurposeExpresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSDDepositorInsertVQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-B3omega1 (GB1694)
Plasmid#160643PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRAF4
Plasmid#58318PurposeEGFP-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
BirA_BVES untagged
Plasmid#196581Purposeexpresses BirADepositorInsertBirA_Ecoli
UseBaculovirus expressionTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-SOX10
Plasmid#134669PurposeRetroviral expression of human SOX10DepositorInsertSRY-box transcription factor 10 (SOX10 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
HSC1-D4Z4-GiP
Plasmid#58542PurposeDerived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3β
Plasmid#111880PurposeMode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: 14-3-3β fused to C-terminal…ExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Atoh1wt-IRES-RFP
Plasmid#33334DepositorInsertAtoh1 (Atoh1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Trib3
Plasmid#131606Purposeexpression of mouse Trib3 in mammalian cellsDepositorInsertTrib3 (Trib3 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3σ
Plasmid#111881PurposeMode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: 14-3-3σ fused to C-terminal…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1p shDna2
Plasmid#31951DepositorInsertshRNA against hDna2 (DNA2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
FLAG-β-arrestin2 RRK/Q
Plasmid#38261DepositorInsertβ-arrestin2 (Arrb2 Rat)
UseTagsFlagExpressionMammalianMutationArg233, Arg237, and Lys251 converted to GlutaminesPromoterCMVAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
KA1396_pGL4-UBCmin-luc2/Klf4-E'
Plasmid#124187PurposeLuciferase reporter driven by the Klf4 enhancerDepositorInsertKlf4 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-FUCCI-Luc
Plasmid#133364PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporterDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsHA-tagged Clover-GemininExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Fbxo7-IRES-GFP
Plasmid#171786Purposeretrovirus productionDepositorInsertFbxo7 isoform 1 (FBXO7 Human)
UseRetroviralTagsExpressionMutationnonePromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-MEG3-del5
Plasmid#44732DepositorInsertMaternally Expressed Gene 3 (MEG3 Human)
UseTagsExpressionMammalianMutationThe first 450 nt were deleted from the MEG3 cDNA.PromoterhCMVAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-SunTag
Plasmid#158410PurposeCRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activationDepositorInsertdpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
RGG-mCherry-RGG-eDHFR
Plasmid#164648PurposeTwo RGG domains tagged with mCherry and eDHFRDepositorInsertRGG
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
KA2626_pGL4-UBCmin-luc2/Klf2-D'
Plasmid#124199PurposeLuciferase reporter driven by the Klf2 enhancerDepositorInsertKlf2 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(T31N)-HA
Plasmid#79405PurposeExpresses C-terminally HA-tagged Arf4(T31N) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsHAExpressionMammalianMutationT31NPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(Q71L)-HA
Plasmid#79404PurposeExpresses C-terminally HA-tagged Arf4(Q71L) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsHAExpressionMammalianMutationGln 71 LeuPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A
Plasmid#127790PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-pHAT
Plasmid#83928PurposehsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagIIDepositorInserthuman Doublecortin isoform b (DCX Human)
UseTags6-His tag and Strep-TagIIExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
KA1401_pGL4-UBCmin-luc2/Klf4-F'
Plasmid#124188PurposeLuciferase reporter driven by the Klf4 enhancerDepositorInsertKlf4 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1395_pGL4-UBCmin-luc2/Klf4-B'
Plasmid#124186PurposeLuciferase reporter driven by the Klf4 enhancerDepositorInsertKlf4 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A
Plasmid#32456DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsFLAGExpressionMammalianMutationS129A, S133A, S137A (S3V, G96R, and P108L also pr…PromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB3042(gRNA X-4)
Plasmid#73284PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3020(gRNA X-2)
Plasmid#73282PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PtW-eSpCas9
Plasmid#133357PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsHA-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4-2 WW2
Plasmid#111883PurposeMode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: NEDD4-2 WW2 fused to C-term…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Atoh1wt-IRES-GFP
Plasmid#33333DepositorInsertAtoh1 (Atoh1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj10a-IRES-EGFP
Plasmid#164947PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFPDepositorInsertkcnj10a (kcnj10a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(WT)-HA
Plasmid#79403PurposeExpresses C-terminally HA-tagged Arf4(WT) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX-B3omega2 (GB1695)
Plasmid#160644PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (omega2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-B2alpha2 (GB1696)
Plasmid#160620PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB3050(gRNA XII-5)
Plasmid#73292PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C49,50,83,103A
Plasmid#58421PurposeExpresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 49, 50, 83, and 103 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-CaMKKbeta S129D, S133D, S137D
Plasmid#32455DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseTagsFLAG and GFPExpressionMammalianMutationS129D, S133D, S137DPromoterCMVAvailable sinceMay 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
p-miR-216a
Plasmid#65054PurposeExpression of miR216a in mammalian cellsDepositorInserthsa-miR-216a (pre-miRNA) (MIR216A Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Atoh1ER-IRES-GFP
Plasmid#33335DepositorInsertAtoh1 (Atoh1 Mouse)
UseRetroviralTagsERExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCfB3041(gRNA X-3)
Plasmid#73283PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170816PurposeLentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-IDepositorInsertNLS-mScarlet-I-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170817PurposeLentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-IDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB3047(gRNA XII-1)
Plasmid#73289PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcna1a-IRES-EGFP
Plasmid#164965PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFPDepositorInsertkcna1a (kcna1a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK-HA-TRAF4
Plasmid#58314PurposeHA-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -