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Plasmid#105813PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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KA2880_pPB-TREtight-FLAG-Esrrb-InO
Plasmid#124178PurposepiggyBac-based Dox-inducible expression of FLAG-EsrrbDepositorInsertFLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-UMPS (Y37A, R155A)-3xFLAG
Plasmid#87976PurposeExpression of UMPS (Y37A, R155A) in mammalian cellsDepositorInsertUMPS (Y37A, R155A) (UMPS Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationY37A, R155APromoterCMVAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag CKM
Plasmid#20458DepositorInsertCKM (CKM Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)
Plasmid#87202PurposeTo express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
UseTagsFLAG and GFPExpressionMammalianMutationResidues from 90-107 are inserted.PromoterCMVAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 3XFlag ATXN1[15Q]-delAXH
Plasmid#33241DepositorInsertATXN1[2Q]-delAXH (ATXN1 Human)
UseTags3xflagExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag RIOK3
Plasmid#20618DepositorInsertRIOK3 (RIOK3 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PACSIN1
Plasmid#20545DepositorInsertPACSIN1 (PACSIN1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag p38 alpha(agf)
Plasmid#20787DepositorInsertpCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationreplaced Thr180 and Tyr182 with Ala and Phe, resp…PromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGGEV_4'_+nls-mCherry-Flag-_+1_BK+
Plasmid#49313PurposeGolden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codonDepositorInsertnls-mCherry-Flag
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBB FLAG XIAP D148A/W310A
Plasmid#25685DepositorInsertXIAP (XIAP Human)
UseTagsFLAGExpressionMammalianMutationD148A W310APromoterAvailable sinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS
Plasmid#106355PurposeCRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locusDepositorInsertMYOD1 gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag BMX
Plasmid#20431DepositorInsertBMX (BMX Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25
Plasmid#111743PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ25 (polyQ repeat)PromoterCMV and P10Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D-CD4-FLAG
Plasmid#51601Purposeexpresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4DepositorInsertSema4D/CD4-FLAG (Sema4d Human, Mouse)
UseTagsFLAG and mycExpressionMammalianMutationSema4D amino acids 660-861 were replaced with CD4…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 212-364
Plasmid#78795PurposeTo overexpress TIP60 212-364 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutation212-364, G214APromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PMVK
Plasmid#20593DepositorInsertPMVK (PMVK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag CAMKK1
Plasmid#20442DepositorInsertCAMKK1 (CAMKK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only