-
Plasmid#121848PurposepMVP destination vector, empty Lentivirus vector backboneDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only
-
pCIG3 N
Plasmid#132941PurposeMLV packaging plasmid for N-tropic MLV gag-pol expressionDepositorInsertN-tropic MLV gag-pol
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-3UAG
Plasmid#82501PurposeExpresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153DepositorInsertGreen Fluorescence Protein with 3 UAG codons
UseTagsExpressionBacterialMutationAdded an UAG codon at the amino acid position 3, …PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_APEX_FLAG_Ct
Plasmid#194069Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-APEX-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA-H2B-mScarlet3
Plasmid#224436PurposeConstruct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3DepositorInsertH2B-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMV & SP6Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1273
Plasmid#142774PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDPF2 (DPF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTF101.2
Plasmid#134771PurposeA binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorInsertXBP1 (XBP1 Human)
UseTagsFlagExpressionMammalianMutationsplicedPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN6
Plasmid#203684PurposeExpresses red fluorescent marker DsRed-Express in Listeria monocytogenesDepositorInsertDsRed-Express
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2849
Plasmid#74112PurposeEnhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.DepositorInsertminiSOG(Q103L)
UseGateway topo cloningTagsPHExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTetO-ISce1-TetO
Plasmid#58506PurposeISce1 restriction site is flanked by Tet operator repeats, 96 on each sideDepositorInsertTet repeats flanking ISce1 restriction site
UseTagsExpressionBacterialMutationPromoterno promoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2RY2-Tango
Plasmid#66474PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertP2RY2 (P2RY2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT28-proD/SaEGFP
Plasmid#120321PurposeBacterial eGFP labelingDepositorInserteGFP with Staphylococcus aureus codon optimization
UseTagsExpressionBacterialMutationPromoterproDAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2678
Plasmid#144065PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPEG3 (PEG3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Sharpin
Plasmid#195092PurposeGene Overexpression of Human SHARPINDepositorInsertSHARPIN (SHARPIN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e(TadA-8e V106W)
Plasmid#138508PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-dLbCas12a
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGlnA-Ypet
Plasmid#98278Purposemammalian expression of GlnA-YpetDepositorInsertGlnA
UseTagsYpetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU+UIM, aa 287-481)
Plasmid#61406PurposeExpresses human OTUD1 (OTU+UIM domains) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0042-PguCas13b crRNA backbone
Plasmid#103853PurposeFor cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorInsertU6-PguCas13b DR-BbsI-BbsI-polyT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Multiplex-CRISPR-miR-200FAM-T2A-GFP
Plasmid#179856Purposeto delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200bDepositorInsertUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only