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Plasmid#45874DepositorInsertSemcap3 in situ probe (Pdzrn3 Mouse)
UseIn situTagsExpressionMutationcontains bp# 3184-3805 of AF127085.1PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR8-DJ1-D149A
Plasmid#29355DepositorInsertDJ1 (PARK7 Human)
UseEntry vectorTagsExpressionMutationAsp149AlaPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Inhba
Plasmid#45868DepositorInsertInhba in situ probe (Inhba Mouse)
UseIn situTagsExpressionMutationnt 428-933 from Inhba mRNA (NM_008380.1)PromoterAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat-ADE2
Plasmid#232106PurposeExpresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterZ3Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Hyg-ADE2
Plasmid#232107PurposeExpresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterZ3Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan
Plasmid#232099PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat
Plasmid#232097PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Hyg
Plasmid#232098PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-SF14P
Plasmid#209448Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from SF14P promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-kasOP*
Plasmid#209447Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from kasOP* promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCRbluntII-pHES7-STOPgRNA
Plasmid#204349PurposegRNA to target pig HES7 stop codonDepositorInsertPig HES7-STOP-gRNA (HES7 Sus domesticus (pig))
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one_Bip gRNA1
Plasmid#183696PurposeExpresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selectiveDepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-DrSmg1_F
Plasmid#146277PurposeBacterial Expression of DrSmg1DepositorInsertDrSmg1 (smg1 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-DrSmg5_F
Plasmid#146278PurposeBacterial Expression of DrSmg5DepositorInsertDrSmg5 (smg5 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-DrSmg6_F
Plasmid#146279PurposeBacterial Expression of DrSmg6DepositorInsertDrSmg6 (LOC100334020 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-DrSmg7_F
Plasmid#146280PurposeBacterial Expression of DrSmg7DepositorInsertDrSmg7 (smg7 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-DrUPF1_F
Plasmid#146281PurposeBacterial Expression of DrUPF1DepositorInsertDrUPF1 (upf1 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-DrUPF2_F
Plasmid#146282PurposeBacterial Expression of DrUPF2DepositorInsertDrUPF2 (upf2 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only