Addgene

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pAN-PA3-RFP

Plasmid

#62249

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pA3

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTK713

Plasmid

#114699

Purpose

Express NuMA(1-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115ΔNLS)-RFP-Nano

Use

Tags

Expression

Mutation

Promoter

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pWT060e

Plasmid

#107905

Purpose

W2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-sgRNA B (CCR5)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTK729

Plasmid

#114710

Purpose

Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-705+1700-2115ΔNLS)-RFP-Nano

Use

Tags

Expression

Mutation

Promoter

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_2

Plasmid

#63803

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pWT019b

Plasmid

#107894

Purpose

W1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-YFP

Plasmid

#62246

Purpose

used to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_52

Plasmid

#63808

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBad_crtEBI_37

Plasmid

#63807

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-OR

Plasmid

#62307

Purpose

synthetic circuit: OR gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A4NT sgRNA
A2NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PA2, PPhlF, and pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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PMT-mApple_2

Plasmid

#203772

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

PMT-mApple dimer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-3xGFP-tDeg

Plasmid

#191371

Purpose

Overexpression of 3xGFP-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

3xGFP-tDeg

Use

Tags

3xGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-Halo-tDeg

Plasmid

#191370

Purpose

Overexpression of Halo-tDeg in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Insert

Halo-tDeg

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSth_Cas8

Plasmid

#178776

Purpose

Expression of S. thermophilus type I-E cas8

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas8

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSth_Cse2

Plasmid

#178777

Purpose

Expression of S. thermophilus type I-E cse2

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cse2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSth_Cas5

Plasmid

#178778

Purpose

Expression of S. thermophilus type I-E cas5

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSth_Cas7

Plasmid

#178780

Purpose

Expression of S. thermophilus type I-E cas7

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEh_Cas6

Plasmid

#178756

Purpose

Expression of E. haloalkaliphila type I-E cas6

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

E. haloalkaliphila type I-E cas6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEh_Cas7

Plasmid

#178757

Purpose

Expression of E. haloalkaliphila type I-E cas7

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

E. haloalkaliphila type I-E cas7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMs_Cas8

Plasmid

#178759

Purpose

Expression of Marinomonas sp. type I-E cas8

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Marinomonas sp. type I-E cas8

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge