Showing: 441 - 460 of 581 results
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Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionTagsExpressionMutationPromoterPol II readthrough transcription after integrationAvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-mNG-NL
Plasmid#169106PurposeExpresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - mNeonGreen - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-mNG
Plasmid#195160Purposemammalian expression of siRNA-resistant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
UseTagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
siRNA resistant ABHD16A-S176AS306A-mNG
Plasmid#195165Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
UseTagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-H189AD194A-mNG
Plasmid#195166Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
UseTagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant ABHD16A-S355A-mNG
Plasmid#195167Purposemammalian expression of siRNA-resistant mutant ABHD16A tagged with mNeonGreenDepositorInsertABHD16A (ABHD16A Human)
UseTagsmNeonGreenExpressionMammalianMutationWobble mutations for siRNA resistance from L90 to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-CAG-mNG2(1-10)
Plasmid#206042PurposeRepair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.DepositorInsertAAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
UseCRISPR and Synthetic Biology; Donor templateTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRB116.2
Plasmid#181946PurposeaTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromoterTorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106AvailabilityAcademic Institutions and Nonprofits only -
pRB120.2
Plasmid#181947PurposeaTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromotermNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106AvailabilityAcademic Institutions and Nonprofits only -
pRB113.2
Plasmid#181948PurposeaTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromoterNarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertUseTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106AvailabilityAcademic Institutions and Nonprofits only -
pRB133.2
Plasmid#181950PurposeaTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirKDepositorInsertPhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
UseTagsmNeonGreenExpressionBacterialMutationPromoterPhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-mNG-eDHFR(69K6)
Plasmid#178853PurposeMammalian expression of mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alphaAvailabilityAcademic Institutions and Nonprofits only -
pPEE47
Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
UseTagsExpressionBacterial and YeastMutationPromoterpGAL1(yeast) and pT7-RNAP(bacteria)AvailabilityAcademic Institutions and Nonprofits only -
pN1-COX8-mNG
Plasmid#129342PurposeMitochondria matrix localizationDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pN1-TOMM20-mNG
Plasmid#129347PurposeMitochondrial outer membrane localizationDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B4
Plasmid#172845PurposeCRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mNeonGreen
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mNG-eDHFR(69K6)-p85iSH2
Plasmid#172103PurposeMammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)DepositorInsertmNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-GFP
Plasmid#186606PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFPDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 441 - 460 of 581 results