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Plasmid#173229PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro
Plasmid#173230PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro
Plasmid#173231PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO
Plasmid#205463PurposeA backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-A; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO
Plasmid#205465PurposeA backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-C; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-B-UGI-PURO
Plasmid#205464PurposeA backbone containing a RsDddA fragment B for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-B; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-D-UGI-PURO
Plasmid#205466PurposeA backbone containing a RsDddA fragment D for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-D; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro
Plasmid#173232PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1333N)-UGI-Puro
Plasmid#173233PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1397C)-UGI-Puro
Plasmid#173234PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1397N)-UGI-Puro
Plasmid#173235PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRTagsExpressionMammalianMutationPromoterPgkAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVU/myc
Plasmid#24179DepositorInsertChloramphenicol resistance gene (CmR) - ccdB gene
UseLentiviral; GatewayTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDESTP10aw
Plasmid#41439DepositorInsertccdB (ccdB )
UseTagsExpressionMutationPromoterAvailable sinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVU/RED
Plasmid#24178DepositorInsertChloramphenicol resistance gene (CmR) - ccdB gene
UseLentiviral; GatewayTagsDsREDExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorUseTagsA177CExpressionMutationPromoterpBAD and pLacAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyTagsExpressionMutationBsaI site removedPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD288
Plasmid#137017PurposemNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-GLO^SEC^3xFlag + ccdB
UseCRISPR and Cre/LoxTags3xFlag and C. elegans GLO-optimized mNGExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD291
Plasmid#137019PurposemKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmKate2-GLO^SEC^3xFlag + ccdB
UseCRISPR and Cre/LoxTags3xFlag and C. elegans GLO-optimized mKate2ExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only