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Plasmid#138312PurposeLenti expression of AcrIIA14, C-terminusDepositorInsertAcrIIA14, C terminus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-3
Plasmid#112843Purposetransient/stable overexpression of the C-terminal domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationC2 domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-1
Plasmid#112841Purposetransient/stable overexpression of the N-terminal domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationN domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
KaiC CI cat- (E77Q;E78Q)
Plasmid#41886DepositorInsertKaiC CI cat -(E77Q;E78Q)
UseTagsExpressionBacterialMutationchanged glutamic acid 77 and 78 to glutaminePromotert7 promoterAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
KaiC CII cat- (E318Q)
Plasmid#41887DepositorInsertKai C CII cat (E318Q)
UseBl de3 21TagsExpressionBacterialMutationchanged amino acid 318 E to QPromotert7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE74 TM Beta-catenin 1-9 in CS2+
Plasmid#16832DepositorInsertTM beta-catenin 1-9 (ctnnb1.L Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
GFP-alpha-E-Catenin 3xFP4 (895)
Plasmid#67936PurposeMammalian expression of alpha E cateninDepositorInsertalpha E catenin (Ctnna1 Mouse)
UseTags3xFP4 and EGFPExpressionMammalianMutationN-terminal domainPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-SOD2-2A-Catalase-WPRE
Plasmid#67635PurposeAAV vector expressing both SOD2 and mitochondrial targeted CatalaseDepositorUseAAVTagsExpressionMutationDeleted the Peroxisome Targeting Signal from Cata…PromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH N90-beta-catenin
Plasmid#86499PurposeMammalian expression of Myc tagged N90 beta-cateninDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsMycExpressionMammalianMutationDeletion of amino acids 1-90; constitutively acti…PromoterEF1aAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry
Plasmid#181851PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.DepositorUseTagsmCherryExpressionMammalianMutationLysine 128 in the DnaJB1-PKAcat fusion changed to…PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)
Plasmid#171089PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsDecoy sgRNA
dCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationD10A, H840APromoterTUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro shRNA beta-catenin
Plasmid#18803Purpose3rd gen lentiviral vector for knocking down beta-catenin gene expressionDepositorInsertb-catenin shRNA (CTNNB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJuly 22, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus
Plasmid#105127PurposeUsed along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent proteinDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromotercryaa (crystallin) and fabp10aAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-BX SpyCatcher003-(short)mCD80
Plasmid#210567PurposeExpresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domainDepositorInsertSpycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp YFP-PIF-SOS2cat
Plasmid#50851PurposeOptogenetic Ras activation system; cytoplasmic componentDepositorInsertsUseLentiviralTagsYFPExpressionMutationresidues 566–1049PromoterAvailable sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-beta-catenin-IRES-GFP
Plasmid#14717DepositorInsertbeta-catenin (Ctnnb1 Mouse)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
123_pAAV-ProC1-CatCh-GFP-WPRE
Plasmid#125937PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProC1Available sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only