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Showing: 441 - 460 of 463 results

  5. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

    Plasmid
    #216733
    Purpose
    Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase and sgCTG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    U6 and CAG
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216733-plasmid-map-sequence-id-430318

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  15. pUt-mNF-Cas13d

    Plasmid
    #224789
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224789-plasmid-map-sequence-id-445954

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  19. pMXS-IRES-Blast HA-GPAT4-ΔNs5

    Plasmid
    #173169
    Purpose
    Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site
    Depositor
    Kivanc Birsoy
    Article
    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi
    Insert
    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    Synonymous mutations at sgRNA sites, amino acids …
    Promoter
    MMLV
    Available since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173169-plasmid-map-sequence-id-341378

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  20. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

    Plasmid
    #173167
    Purpose
    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation
    Depositor
    Kivanc Birsoy
    Article
    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi
    Insert
    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    Use
    Retroviral
    Tags
    3XFLAG
    Expression
    Mutation
    Synonymous mutations at sgRNA sites, G2A, S6A
    Promoter
    MMLV
    Available since
    Aug. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    173167-plasmid-map-sequence-id-341376

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Showing: 441 - 460 of 463 results