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Plasmid#187693PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa139-564PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-D (aa160-340)-EGFP
Plasmid#187696PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa160-340PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-B (aa309-564)-EGFP
Plasmid#187694PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa309-564PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Profiline
Plasmid#89925PurposeBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actinDepositorInsertprofiline-1 (PFN1 Human)
UseTagsNoneExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-C
Plasmid#118367PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-N
Plasmid#118368PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-N
Plasmid#118366PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FlagExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-C
Plasmid#118369PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-mDia1(FH1FH2DAD)-His
Plasmid#85822Purposecontains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.DepositorInsertProtein diaphanous homolog 1 (Diaph1 Mouse)
UseTags8*His and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cytb5
Plasmid#182579PurposeExpresses mCherry targeted to the ER via the CytB5RR tail anchor sequenceDepositorInsertmCherry-Cytb5
UseTagsCytb5ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHyVec5
Plasmid#34792DepositorTypeEmpty backboneUseHydra expressionTagsActin/RFP fusion proteinExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHyVec1
Plasmid#34789DepositorTypeEmpty backboneUseHydra expressionTagsActin/GFP fusion proteinExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB403-Lifeact-Citrine
Plasmid#100582PurposeBinary vector for expression of Citrine-fused Lifeact (Lifeact-Citrine) to visualize actin filaments in plantsDepositorInsertLifeact-Citrine
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP
Plasmid#58470PurposeExpresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoterDepositorInsertLifeact
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB105-mTalin
Plasmid#100584PurposeBinary vector for expression of Citrine-fused mouse Talin (Citrine-mTalin) to visualize actin filaments in plantsDepositorInsertCitrine-mTalin (Tln1 Mouse)
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
UseTagsFis1ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-BirA*-pDEST-N-pcDNA5/FRT/TO
Plasmid#118375PurposeExpression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.DepositorTypeEmpty backboneUseDestinationTagsHA-BirA*ExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-GFP-Fis1
Plasmid#182576PurposeExpresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertLifeAct-GFP-Fis1
UseTagsFis1 and LifeActExpressionMammalianMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-GFP-cytoB5RR
Plasmid#182577PurposeExpresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequenceDepositorInsertLifeAct-GFP-cytoB5RR
UseTagsLifeAct and cytoB5RRExpressionMammalianMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-Paxillin-22
Plasmid#54935PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorInsertPaxillin
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only