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Plasmid#118368PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only
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Flag-GFP11-pDEST-N
Plasmid#118366PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FlagExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-C
Plasmid#118369PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Profiline
Plasmid#89925PurposeBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actinDepositorInsertprofiline-1 (PFN1 Human)
UseTagsNoneExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cytb5
Plasmid#182579PurposeExpresses mCherry targeted to the ER via the CytB5RR tail anchor sequenceDepositorInsertmCherry-Cytb5
UseTagsCytb5ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHyVec5
Plasmid#34792DepositorTypeEmpty backboneUseHydra expressionTagsActin/RFP fusion proteinExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHyVec1
Plasmid#34789DepositorTypeEmpty backboneUseHydra expressionTagsActin/GFP fusion proteinExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB403-Lifeact-Citrine
Plasmid#100582PurposeBinary vector for expression of Citrine-fused Lifeact (Lifeact-Citrine) to visualize actin filaments in plantsDepositorInsertLifeact-Citrine
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Lifeact-EGFP
Plasmid#58470PurposeExpresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoterDepositorInsertLifeact
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB105-mTalin
Plasmid#100584PurposeBinary vector for expression of Citrine-fused mouse Talin (Citrine-mTalin) to visualize actin filaments in plantsDepositorInsertCitrine-mTalin (Tln1 Mouse)
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
UseTagsFis1ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-mDia1(FH1FH2DAD)-His
Plasmid#85822Purposecontains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.DepositorInsertProtein diaphanous homolog 1 (Diaph1 Mouse)
UseTags8*His and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-BirA*-pDEST-N-pcDNA5/FRT/TO
Plasmid#118375PurposeExpression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.DepositorTypeEmpty backboneUseDestinationTagsHA-BirA*ExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-GFP-Fis1
Plasmid#182576PurposeExpresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertLifeAct-GFP-Fis1
UseTagsFis1 and LifeActExpressionMammalianMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-GFP-cytoB5RR
Plasmid#182577PurposeExpresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequenceDepositorInsertLifeAct-GFP-cytoB5RR
UseTagsLifeAct and cytoB5RRExpressionMammalianMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple-Paxillin-22
Plasmid#54935PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorInsertPaxillin
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
KAT14-pDEST10
Plasmid#118382PurposeHIS-tagged insect cell expression vector with KAT14.DepositorInsertKAT14 (KAT14 Human)
UseTagsHISExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-DSep1
Plasmid#174493Purposebacterial expression of Drosophila Sep1DepositorInsertSep1 (Sep1 Fly)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICPIS-CB
Plasmid#73356PurposeAdenoX shuttle vector with chicken-beta actin CMV enhancer promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterchicken beta-actin CMV enhancerAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-HsACTG1*-thymosinB-8His
Plasmid#111147PurposeExpresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.DepositorInsertactin gamma 1 (ACTG1 Human)
UsePichia pastoris integration vectorTagsThymosin beta and a His-tagExpressionYeastMutationcodon-optimised for expression in Pichia pastorisPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only