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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and mouse U6

Available since

Availability

Academic Institutions and Nonprofits only

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LentiV2-EF1α-nCas9/RT

Plasmid

#210188

Purpose

Expression of nCas9/RT-P2A-Puro

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

nCas9/RT-P2A-Puro

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti_MS2_P65_HSF1_2A_puro

Plasmid

#118699

Purpose

To express MS2-P65-HSF1

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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SpRY Cas9

Plasmid

#188491

Purpose

Expresses FLAG tagged SpRY Cas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMCB619

Plasmid

#171011

Purpose

CRISPR sgRNA vector (puro-BFP with BstXI/BlpI digest sites).

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

U6:sgRNA_Puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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SpRY dCas9 KRAB

Plasmid

#188501

Purpose

Expresses FLAG tagged SpRY dCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

EF1a

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-ATG5

Plasmid

#99573

Purpose

Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting ATG5 (ATG5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9-MSK1

Plasmid

#165601

Purpose

Expresses Sp dCas9 fused to human MSK1

Depositor

Isaac Hilton

Article

Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

Insert

ribosomal protein S6 kinase A5 (RPS6KA5 Synthetic, Human, S. Pyogenes)

Use

CRISPR and Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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SpRY dCas9 VPR

Plasmid

#188511

Purpose

Expresses FLAG tagged SpRY dCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgULK1-1

Plasmid

#109004

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ULK1-sgRNA #1 (ULK1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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sgp27#1

Plasmid

#102759

Purpose

Expresses sgRNA targeting mouse p27

Depositor

Jin Chen

Article

Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

Insert

p27 (Cdkn1b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-2

Plasmid

#109009

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #2 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSCL.040

Plasmid

#184974

Purpose

Test effect of extending a1/a2 on ADE2 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2

Use

Tags

Expression

Yeast

Mutation

ADE2 donor P272stop, a1/a2 length extended to 27 …

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e VPR

Plasmid

#188516

Purpose

Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-VPR

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD2KN0895

Plasmid

#177091

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQDPKN0892

Plasmid

#177089

Purpose

MoClo Level P, position 1, plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2GFP

Plasmid

#82416

Purpose

3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Has Service

Cloning Grade DNA

Insert

GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EFS (P2A)

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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