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Plasmid#12152DepositorInsertRhoA Biosensor T19N (RHOA Human)
UseTags6xHisExpressionBacterial, Insect, and Mamm…MutationRhoA T19N (dominant-negative mutation)*PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-12aa-mCit
Plasmid#53237PurposeFluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags12aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor
Plasmid#12153DepositorInsertRhoA Biosensor (RHOA Human)
UseRetroviralTags6XHisExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
GTH-y
Plasmid#81105PurposeGene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTL-y
Plasmid#81100PurposeGene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTT-y
Plasmid#81110PurposeGene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB425
Plasmid#68690PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTU-y
Plasmid#81095PurposeGene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTK3_017
Plasmid#123732PurposeEncodes Fluorescent probe for G1/S transition as a Type 3 part to be used in the MTK systemDepositorInsertCitrine::Geminin(1-110)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38-AtLEA4-5
Plasmid#178189PurposeSED1 biosensor for expression in Saccharomyces cerevisiaeDepositorInsertSED1 biosensor
UseTagsExpressionYeastMutationPromoterpPMA1Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHBS1449 SED1-Lenti
Plasmid#180824PurposeSED1 biosensor for stable integration and expression in mammalian cellsDepositorInsertSED1 osmosensor
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HisMBP-SED1
Plasmid#178191PurposeSED1 biosensor for expression in Escherichia coliDepositorInsertSED1 biosensor
UseTags6xHis-MBPExpressionBacterialMutationPromotertacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-nlsSED1
Plasmid#178190PurposeSED1 biosensor for expression in Arabidopsis thalianaDepositorInsertSED1 biosensor
UseTagsSV40 NLSExpressionPlantMutationPromoterpUBQ10Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAM66
Plasmid#86478PurposeExpresses p9-mCitrineDepositorInsertp9-mCitrine
UseSynthetic BiologyTagsmCitrineExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCAM12
Plasmid#86471PurposeExpresses p12 and p9-mCitrineDepositorInsertp12, p9-mCitrine
UseSynthetic BiologyTagsmCitrineExpressionBacterialMutationPromoterpBADAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAM53
Plasmid#86477PurposeExpresses p12 and mCitrine-p9DepositorInsertp12, mCitrine-p9
UseSynthetic BiologyTagsmCitrineExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
JD180
Plasmid#229486PurposeAdenoviral helper plasmid containing inducible E2A and L4-22k/33k genesDepositorInsertE2A, L4-22k/33k, mCitrine
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterTre (E2A, L4-22k/33k), ETR (mCitrine)Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC230
Plasmid#118871Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mCitrine, cl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM29
Plasmid#135489PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCitrineDepositorInserthph SpunH2A/Bpr hph-mCitrine
UseTagsmCitrineExpressionBacterial and YeastMutationPromoterSpunH2A/Bpr (divergent)Available sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS1422
Plasmid#63793PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection markerDepositorInserteCitrine-Twin-tag followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Twin-tagExpressionMutationPromoternoneAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only