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Plasmid#210332PurposeOverexpression of rTOM20DepositorInsertrTOM20 (Tomm20 Rat)
UseTagsTagBFPExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP
Plasmid#214367PurposeExpression of the Blue Fluorescent ProteinDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-PLCδ-PH
Plasmid#214275PurposeMammalian expression of blue fluorescent PI(4,5)P2 reporter mTagBFP2-PLCδ-PHDepositorArticleInsertmTagBFP2-PLCδ(1-175) (PLCD1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
H_NES 41kDa (BFP)
Plasmid#201393PurposeNucleocytoplasmic transport probeDepositorInsertHIV_NES-BFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
H_NLS 41kDa (BFP)
Plasmid#201391PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40-BFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 41kDa (BFP)
Plasmid#201392PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-BFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-shBACH1_BFP
Plasmid#199217PurposeExpression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone.DepositorInsertshBACH1 (BACH1 Synthetic)
UseRNAiTagsExpressionMutationPromoterhuman U6Available sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKozak_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199495PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_0_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199478PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199479PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199480PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH FRB-BFP
Plasmid#202425PurposeTagBFP FKBP-rapamycin binding (FRB) dimerization domainDepositorInsertFKBP-rapamycin binding (FRB) dimerization domain
UseLentiviralTagsTagBFPExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-Mss4D636K-Kina
Plasmid#202725Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-Mss4-Kina
Plasmid#202724Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:S. cerevisiae Mss4(377-756) (MSS4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-ccdB-EBFP2
Plasmid#201137PurposepLEX_307 plasmid with EBFP2 markerDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-BFP
Plasmid#190259PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertBFP
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-R60f-BFP
Plasmid#186608PurposeEncodes human Reticulon 2 disease mutant R60f fused to BFPDepositorInsertHuman Reticulon 2 mutant R60f (RTN2 Human)
UseTagsBFPExpressionMammalianMutationc.178insC (R60f)PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
sTag-BFP-6xHIS
Plasmid#186905PurposeBacterial expression of soluble protein tagBFP-sfCherry11-SpyTag with a 6xHIS tagDepositorInsertSpyTag003-sfCherry11-tagBFP-6xHIS
UseTagstagBFP, 6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-BFP-NES
Plasmid#184461PurposeCytosolic EBFP2 expression in mammalian cellsDepositorInsertEBFP2-NES
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pODN-H2BC11-TagBFP
Plasmid#183867PurposeRepair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.DepositorInsertH2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
UseCRISPR; Donor templateTagslinker-mTagBFPExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only