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LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

Plasmid

#192376

Purpose

To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-uORF-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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ppih.001.177.W133A

Plasmid

#137655

Purpose

Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Melissa Jurica, Tara Davis

Article

Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

Insert

ppih (PPIH Human)

Use

Tags

GST-(His)6-thrombin

Expression

Bacterial

Mutation

001-177-W133A

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL706

Plasmid

#140376

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 6BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-6BP-tetO-3WJdB-T

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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Thy1.1-muSOX D219A

Plasmid

#131704

Purpose

encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

Thy1.1-muSOX

Use

Tags

Expression

Mammalian

Mutation

Aspartic acid 219 mutated to an alanine

Promoter

CMV

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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rs676-minor

Plasmid

#113376

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs67668514 minor allele (-)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs67668514 minor allele

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE/SP S245X with 5'UTR

Plasmid

#92376

Purpose

Inducible expression of S245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

S245X

Promoter

Tet-responsive P tight

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00230

Plasmid

#34376

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8388 (CG8388 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAcUW51-CgE

Plasmid

#13762

Depositor

Pamela Bjorkman

Article

Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

Insert

HSV-1 glycoprotein E

Use

Tags

His

Expression

Insect

Mutation

C-terminal region of the HSV-1 gE (KOS strain ect…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
px459-TDP-43 sgRNA1

Plasmid

#133762

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Talin-TS

Plasmid

#83376

Purpose

FRET probe for measuring tension on Talin molecule

Depositor

Martin Schwartz

Article

Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

Insert

Talin1 (Tln1 Mouse)

Use

Retroviral

Tags

EGFP-F40-tagRFP (tension sensor module)

Expression

Mammalian

Mutation

Tension sensor module is inserted between V447 an…

Promoter

CMV

Available since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-BclXL-pEGFP-C1

Plasmid

#177408

Purpose

To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-XL

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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PET-HiFi SpCas9-NLS-6xHis

Plasmid

#207376

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-C

Plasmid

#53769

Purpose

mammalian expression of flag-tagged ATR C-terminal fragment

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain, aa 1398-2644*

Promoter

CMV

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pHM123DEnv

Plasmid

#137668

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a2(apf)

Plasmid

#13761

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2(apf) (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif THr(183)-Pro-T…

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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px459-TDP 43 sgRNA2

Plasmid

#133763

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-N

Plasmid

#53767

Purpose

mammalian expression of flag-tagged ATR N-terminal fragment

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

N-terminal domain, aa 1-1409

Promoter

CMV

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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CBP TAZ1(340-439)

Plasmid

#173760

Purpose

bacterial expression of mouse CBP TAZ1 domain

Depositor

Peter Wright

Article

De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

Insert

CBP TAZ1 (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ATR-M

Plasmid

#53768

Purpose

mammalian expression of flag-tagged ATR middle region

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

Insert

ATR (ATR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

middle domain, aa 593-1700*

Promoter

CMV

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only
pBZcsx27

Plasmid

#89900

Purpose

Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

csx27

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lac

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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