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Plasmid#110851PurposeLentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)DepositorInsertCas9-VQRRA
UseLentiviralTagsFLAGExpressionMutationD1135V, R1335Q, T1337R and NLS sequence at the N-…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3-P2A-GFP-PGK-Puro
Plasmid#110867PurposeLentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#707
Plasmid#110911PurposeExpresses the C. elegans sup-1(e995) minigene in ventral nerve cord cholinergic motor neuronsDepositorInsertsup-1 minigene
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#865
Plasmid#110912PurposeCoding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacersDepositorInsertAuxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#883
Plasmid#110913PurposeCoding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a proteinDepositorInsert5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-αIFNα-ib
Plasmid#110798PurposeExpression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cellsDepositorInsertintrabody against mouse interferon alpha
UseTagsER retention signal (SEKDEL), ER signal peptide, …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK301
Plasmid#110593PurposeBTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)DepositorInsertBba_B0015 Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQC MCS IRES G418
Plasmid#110344Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Geneticin selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
B. fragilis mRNA toehold switch sensor
Plasmid#110706PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. fragilis species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro
Plasmid#110831PurposeExpresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.DepositorInsertAzgp1 (Azgp1 Mouse)
UseNon-viral gene expression vectorTagsdTomatoExpressionMutationPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-P5CR
Plasmid#110295PurposeExpresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)DepositorInsertPyrroline-5-carboxylate Reductase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
RIPK2 (RIPK2A-c036)
Plasmid#110268PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertRIPK2 (RIPK2 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.puro_shHuR 3UTR
Plasmid#110414PurposeTRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, puromycin selectionDepositorInsertELAVL1 (HuR)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK620
Plasmid#110620PurposeBTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoterDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK621
Plasmid#110621PurposeBTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoterDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK619
Plasmid#110619PurposeBTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoterDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
KLHL3 (KLHL3A-c003)
Plasmid#110251PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKLHL3 (KLHL3 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2_shGLS
Plasmid#110319PurposeshGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2DepositorInsertGLS Glutaminase (GLS Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW8_6XFLAG
Plasmid#110235PurposeCre/Lox C-terminal tagging construct encoding 6 copies of the FLAG epitopeDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAO1_G-alpha
Plasmid#110013PurposeProtein expression and purification of GNAO1_G-alphaDepositorInsertGNAO1_G-alpha (GNAO1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only