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Plasmid#162495PurposeExpresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
UseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac(5A)
Plasmid#162493PurposeExpresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
UseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-EGFP
Plasmid#65278Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-GFP-Numb-S276D
Plasmid#133752PurposeNumb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLDDepositorInsertNUMB endocytic adaptor protein (Numb Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationserine 276 converted to aspartic acidPromoterEF1-alphaAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG-cTP-TurboID-GFP
Plasmid#209403PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in plantaDepositorInsertPM-3XHA-TurbOID
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-mTP-TurboID-GFP
Plasmid#209404PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in plantaDepositorInsertmTP-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GFP-TurboID-3XNLS
Plasmid#209400PurposeTransiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmBam_1-140_AG
Plasmid#148815PurposeInsect Expression of DmBam_1-140DepositorInsertDmBam_1-140 (bam Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-PKCa-C1
Plasmid#112264PurposeITRACKalpha Donor ConstructDepositorInsertprotein kinase C alpha (PRKCA Bovine)
UseTagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-PKCa-mEGFP-N1
Plasmid#112269PurposeIDOCKSalpha Donor ConstructDepositorInsertProtein Kinase C alpha (PRKCA Bovine)
UseTagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
D28 Cortactin GFP
Plasmid#26723DepositorInsertCortactin (Cttn Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion removed 28 amino acids (aa# 334-361) enc…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
PTPRA_C433S-GFP fusion
Plasmid#139638PurposeGFP-tagged gene expressionDepositorInsertPTPRA_C433S (PTPRA Human)
UseTagsGFPExpressionMammalianMutationC433SPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PTPRA_C723S-GFP fusion
Plasmid#139639PurposeGFP-tagged gene expressionDepositorInsertPTPRA_C723S (PTPRA Human)
UseTagsGFPExpressionMammalianMutationC723SPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
SBP-GFP-Tac-TC
Plasmid#162506PurposeExpresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cellsDepositorInsertGFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
UseTagsGFP and SBPExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PTPRA_deltaD2-GFP fusion
Plasmid#139641PurposeGFP-tagged gene expressionDepositorInsertPTPRA_deltaD2 (PTPRA Human)
UseTagsGFPExpressionMammalianMutationD2 domain deletionPromoterCMVAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PTPRA_deltaD1-GFP fusion
Plasmid#139640PurposeGFP-tagged gene expressionDepositorInsertPTPRA_deltaD1 (PTPRA Human)
UseTagsGFPExpressionMammalianMutationD1 domain deletionPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CLC-EGFP206(5)
Plasmid#193573PurposeExpresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 5 amino acids in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 207-211PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLC-EGFP206(6)
Plasmid#193574PurposeExpresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 6 amino acids in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 207-211PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLC-EGFP206(7)
Plasmid#193575PurposeExpresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 207-211PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only