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pCIneo-RLuc-5BoxB_C

Plasmid

#146091

Purpose

Mammalian Expression of 5BoxB

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

5BoxB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTG5

Plasmid

#149487

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PTetA promoter, mRFP.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTetA

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJ2039_N2only

Plasmid

#140682

Purpose

Control (“C”) lacking sg-3

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Control (“C”) lacking sg-3

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ2040_N2only

Plasmid

#140683

Purpose

Control (“C”) lacking sg-4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Control (“C”) lacking sg-4

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS1katGp-RBS33-TP901-proD-oxyR

Plasmid

#78219

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Use

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCyc-Bem1

Plasmid

#66573

Purpose

Express an additional copy of Bem1, integrated at TRP locus

Depositor

Orion Weiner

Article

Jost et al ACS Synth Biol. 2015 Jun 2.

Insert

Bem1

Use

Tags

Expression

Yeast

Mutation

F99I*(see below)

Promoter

pCyc1

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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VCB1-CKKp

Plasmid

#24597

Depositor

Kevin Truong

Article

Mills et al Protein Sci. 2009 Jun . 18(6):1252-60.

Insert

CB1-CKKp

Use

Tags

Venus

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Aug. 12, 2010

Availability

Academic Institutions and Nonprofits only
fIM1266

Plasmid

#132578

Purpose

pZE1-pLsoxS-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

pLsoxS-mCherry-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLsoxS

Available since

Availability

Academic Institutions and Nonprofits only

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pIM11

Plasmid

#132580

Purpose

pZS2-proD-soxR

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

proD-soxR-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Availability

Academic Institutions and Nonprofits only

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pIM10

Plasmid

#132581

Purpose

pZA4-pLsoxS-mCherry

Depositor

Timothy Lu

Article

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Insert

pLsoxS-mCherry-TermT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLsoxS

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pKD4

Plasmid

#45605

Depositor

Barry L. Wanner

Article

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-kan-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

R6K

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pTNS2

Plasmid

#64968

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

tnsABCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pKD3

Plasmid

#45604

Depositor

Barry L. Wanner

Article

Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

Insert

FRT-cat-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

R6K

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-tdMCP-YFP

Plasmid

#99151

Purpose

Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

Dimeric MS2-coat binding protein

Use

Lentiviral

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP702

Plasmid

#45455

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Use

Tags

Expression

Bacterial

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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TS_pLuxLac

Plasmid

#140426

Purpose

Toggle switch circuit responsive to AHL gradient.

Depositor

Yolanda Schaerli

Article

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Inserts

GFP
LacI
TetR
mCherry

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EJ2GFP-puro

Plasmid

#44025

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ2GFP egfp-based chromosomal break reporter

Use

Tags

Expression

Mammalian

Mutation

insertion of I-SceI, 3 frame stop, and flanking m…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2-wtFAKSPARK2

Plasmid

#195263

Purpose

express wtFAKSPARK2

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

FAKSPARK2 sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

PxylA (B. megaterium)

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TRPC1-FLAG

Plasmid

#24408

Depositor

Craig Montell

Article

Xu et al Cell. 1997 Jun 27. 89(7):1155-64.

Insert

TRPC1 (TRPC1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd5-B5

Plasmid

#122555

Purpose

Block 5 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 17998-25062

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PBCAG-eGFP

Plasmid

#40973

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Inserts

PiggyBac transposon 3' terminal repeats
PiggyBac 5' terminal repeats

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLS-iRFP713

Plasmid

#45468

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDmDERusk

Plasmid

#213702

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCherry-FRB

Plasmid

#25920

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

FRB (MTOR Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

Plasmid

#203908

Purpose

Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4UAS_dGFP_PGK_iRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

5xUAS and PGK

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2E1

Plasmid

#15787

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
CKAR

Plasmid

#14860

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG153 FKBP-CFAST11

Plasmid

#130813

Purpose

Expresses FKBP-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST11

Use

Tags

Myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFLP3

Plasmid

#64946

Purpose

Flp recombinase expression vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

Flp recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSirt6-GFP

Plasmid

#20275

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL-CTIG

Plasmid

#14901

Purpose

3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

Depositor

Inder Verma

Article

Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

Insert

CMV-tTA-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-Tre3G-29xGGGGCC-12xMS2

Plasmid

#99149

Purpose

Encodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

29xGGGGCC 12xMS2 hairpins

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG148 FRB-NFAST

Plasmid

#130812

Purpose

Expresses FRB-NFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FRB-NFAST

Use

Tags

myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHSS6hsILMi20

Plasmid

#102536

Purpose

Helper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)

Depositor

Charalambos Savakis, Michalis Averof

Article

Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

Insert

Minos transposase

Use

Tags

Expression

Mutation

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-Tre3G-47xCAG-12xMS2

Plasmid

#99148

Purpose

Encodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vector

Depositor

Ron Vale

Article

Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

Insert

47xCAG 12xMS2 hairpins

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG241 FKBP-CFAST10

Plasmid

#130814

Purpose

Expresses FKBP-CFAST10 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST10

Use

Tags

Myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNIC28-Bsa4

Plasmid

#26103

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Type

Empty backbone

Use

Tags

His - TEV

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW014-2

Plasmid

#85612

Purpose

Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

ugtP-gRNA.P395T

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
piRFP720-N1

Plasmid

#45461

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRm43GW

Plasmid

#133748

Purpose

Three-fragment Multisite Gateway vector construction for red fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Use

Tags

GFP-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBlueSKMimRNA

Plasmid

#102535

Purpose

Template for synthesis of Minos transposase mRNA

Depositor

Michalis Averof

Article

Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

Insert

Minos transposase mRNA

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR GFP-CaaX

Plasmid

#113020

Purpose

for lentivirus production of GFP-CaaX

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

GFP-CaaX

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTC1

Plasmid

#64951

Purpose

source of TcR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-TcR-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1 Nodamura B2

Plasmid

#19716

Depositor

Christopher Sullivan

Article

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Use

Tags

EGFP

Expression

Mammalian

Mutation

2 silent point mutations

Promoter

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pCS2+FucciOrange

Plasmid

#128427

Purpose

Contains insert allowing in-vivo visualization of Cdk1 protein (G1)

Depositor

Rusty Lansford

Article

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Insert

FucciOrange

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/myc-his A Rv0712 (FGE)

Plasmid

#16132

Purpose

Bacterial expression of Mycobacterium tuberculosis FGE

Depositor

Carolyn Bertozzi

Article

Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

Insert

Formylglycine-generating enzyme

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pVG47

Plasmid

#164705

Purpose

yEVenus under the control of tetOpr

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

tetOpr - yEVenus - CLN2 PEST - ADH1t

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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