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Plasmid#170239PurposeBarcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAPK14 (MAPK14 Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1-P2A-Hygro_Barcode
Plasmid#170221PurposeBarcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-myr-CSK K222R-GFP
Plasmid#83471PurposeLentiviral vector for doxycycline-inducible expression of membrane targeted kinase dead CSK in mammalian cellsDepositorInsertCSK (CSK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationK222R (kinase dead)Promoterdoxycycline-inducible promoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2K7-bsd-UBI-tagRFP-KDEL
Plasmid#114179PurposeLentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ERDepositorInserttagRFP-KDEL
UseLentiviralTagsKDEL retention signal and signal peptideExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
DRH323: pAAV_hSyn-QuasAr2
Plasmid#107705Purposein vivo voltage imagingDepositorInsertQuasAr2
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX NPC1-FLAG-TiD
Plasmid#199748PurposeLentiviral construct to stably express NPC1 TurboIDDepositorInsertNPC1 (NPC1 Human)
UseTagsFlag and TurboIDExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-hGH
Plasmid#182412PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusionDepositorInsertsUseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode
Plasmid#170222PurposeBarcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationR24CPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode
Plasmid#170217PurposeBarcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCASP8 (CASP8 Human)
UseLentiviralTagsV5ExpressionMutationC360APromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W
Plasmid#163138PurposeLentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tagDepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode
Plasmid#170225PurposeBarcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertER (ESR1 Human)
UseLentiviralTagsV5ExpressionMutationY537SPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR
Plasmid#126697Purposedonor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertTagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK6-P2A-Hygro_Barcode
Plasmid#170224PurposeBarcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK6 (CDK6 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSynapsin_NLS_GFP-P2A_SNAP_GluA2
Plasmid#170455PurposeExpresses SNAP tagged GluA2 in neuronal cellsDepositorInsertGluA2 (Gria2 Mouse)
UseLentiviralTagsSNAPExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
LRG/Foxa1 e2.4
Plasmid#105511PurposeLentiviral expression of Foxa1 DNA-binding domain (exon 2) targeting sgRNADepositorInsertFoxa1 (sgRNA, e2.4)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
LZF40: LZF40_Fsyn_Cre
Plasmid#109378PurposeSynaptic transmissionDepositorInsertCre-NCBI
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP
Plasmid#126698Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertsgRNA targeting human SCGB3A2 locus
UseTagsExpressionMammalianMutationPromoterHuman U6Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mal*leu*
Plasmid#21173DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationFirst point mutation at nucleotide 5114 (numberin…PromoterAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mkgDUX4mal*mqg*
Plasmid#21174DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationFirst point mutation at nucleotide 5114 (numberin…PromoterAvailable sinceAug. 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Keratin-17
Plasmid#57629PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertKeratin (KRT18 Human)
UseTagstdEosExpressionMammalianMutationV282LPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1puro-shWillin-B
Plasmid#40886DepositorInsertshWillin-B (FRMD6 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1puro-shWillin-A
Plasmid#40885DepositorInsertshWillin-A (FRMD6 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_E210A
Plasmid#204475Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B E210A mutantDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationE210APromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_R153X
Plasmid#204476Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B R153X mutantDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationR153XPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_V748L
Plasmid#204477Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B V748L mutantDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationV748LPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_G671R/N865K
Plasmid#204478Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B G671R/N865K mutantsDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationG671R / N865KPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_E993A
Plasmid#204479Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B E993A mutantDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationE993APromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP10B_I1038T
Plasmid#204480Purposetransfer plasmid for lentiviral vector production expressing Hs ATP10B I1038T mutantDepositorInsertATP10B (ATP10B Human)
UseLentiviralTagsExpressionMutationI1038TPromoterCMVAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-mADAR2-short
Plasmid#175588Purposemammalian expression of mouse short Adar2, blasticidine selectionDepositorInsertShort Mouse Adar2
UseTagsFlagExpressionMammalianMutationPromotercmvAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN554
Plasmid#177361PurposeExpresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tagDepositorInsertKIF1A (KIF1A Human)
UseTagsLeucine zipper, StrepII tag, and mScarletExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluJo-Y 240-2996
Plasmid#171131PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluSz 3501-4065
Plasmid#171132PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluY WT 2598-2996
Plasmid#171134PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTR AluY-Sz WT 2598-4066
Plasmid#171136PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MAPK9-P2A-Hygro_Barcode
Plasmid#170238PurposeBarcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertMAPK9 (MAPK9 Human)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine
Plasmid#160069PurposeConditionally overexpress collybistin (CB2SH3-) and mCitrineDepositorInsertARHGEF9 (Arhgef9 Rat)
UseAAVTagsHA- in CB2SH3ExpressionMutationPromoterhuman Synapsin promoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCh-623
Plasmid#92266PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cellsDepositorInsertdGW182 6W6A (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced W23…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-695
Plasmid#92263PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cellsDepositorInsertdGW182 PAM2 mut (gw Fly)
UseTagsNHAExpressionInsectMutationThe following point mutations were introduced: EF…PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-660
Plasmid#92259PurposePlasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsNHAExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-665
Plasmid#92254PurposePlasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsGSTExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterEF1AAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax
Plasmid#174820PurposeMammalian expression of SpCas9 PEmax prime editorDepositorInsertPEmax
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-PEmax for IVT
Plasmid#178113PurposeTemplate for in vitro transcription of PEmaxDepositorInsertPEmax
UseTemplate for in vitro transcriptionTagsSV40 bpNLS and c-Myc NLSExpressionMutationDetailed in manuscriptPromoterT7 (inactivated)Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-L
Plasmid#120160PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagseGFPExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mApple-proα2(I)-L
Plasmid#120161PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
UseTagsmAppleExpressionMammalianMutationCol1a2 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C
Plasmid#154196PurposeLentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.DepositorInsertsgRNA-BC1-A, sgRNA-BC1-C
UseLentiviral; Catch barcode activationTagsExpressionMammalianMutationPromoterhU6 and mU6Available sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc-LATS2
Plasmid#66852PurposeExpress Myc-tagged LATS2 in mammalian cellsDepositorInsertLATS2 (LATS2 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
ODD-Luciferase-pcDNA3
Plasmid#18965DepositorInsertODD-Firefly Luciferase (HIF1A Human)
UseTagsExpressionMammalianMutationHIF1a ODD sequence fused in frame to luciferase O…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]
Plasmid#190236PurposeAAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinaseDepositorInsertEGFP
UseAAVTagsF2A, LDLR C-terminal sequence, mRFP1, myristoylat…ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only