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Showing: 1101 - 1150 of 2028 results

  2. S+10subs

    Plasmid
    #167176
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+10R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed A at 112 to T; E at 116 to D; I at 135 to…
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167176-plasmid-map-sequence-id-345188

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  3. S+12subs

    Plasmid
    #167177
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+12R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R; A at 112 to T; E at 116to …
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167177-plasmid-map-sequence-id-334485

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  5. TMPRSS2 g1 + Cas9

    Plasmid
    #153015
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153015-plasmid-map-sequence-id-298676

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  6. TMPRSS2 g4 + Cas9

    Plasmid
    #153018
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153018-plasmid-map-sequence-id-298698

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  7. pMX-Flag-GFP_HOMER2

    Plasmid
    #227953
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    HOMER2 (HOMER2 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227953-plasmid-map-sequence-id-455411

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  8. pLV hMyoD-IRES-dsRed

    Plasmid
    #66631
    Purpose
    Co-expresses human MyoD and dsRed
    Depositor
    Charles Gersbach
    Insert
    human MyoD IRES dsRed (MYOD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hUbc
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66631-plasmid-map-sequence-id-114011

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  9. FUGW-3xHA-KCNJ6-mCherry

    Plasmid
    #197032
    Purpose
    Express 3xHA-tagged human KCNJ6 (Girk2), co-expressed with mCherry, from UbiC promoter
    Depositor
    Ronald Hart
    Insert
    KCNJ6 (KCNJ6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    197032-plasmid-map-sequence-id-386375

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  10. 13SA-csy4

    Plasmid
    #97037
    Purpose
    Spectinomycin resistant plasmid with a CDF origin that expresses Csy4 when IPTG induced, for coexpression with Series 371 vectors
    Depositor
    Chris Jeans
    Insert
    Cys4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97037-plasmid-map-sequence-id-182491

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  12. WNK2 (WNK2A-c003)

    Plasmid
    #110250
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    WNK2 (WNK2 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110250-plasmid-map-sequence-id-212284

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  14. pCF806-shABC2

    Plasmid
    #186714
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    186714-plasmid-map-sequence-id-382420

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  15. pCF806-shABC1

    Plasmid
    #186713
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    186713-plasmid-map-sequence-id-376087

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  19. FUGW-APOE-E3-T2A-mCherry

    Plasmid
    #190808
    Purpose
    Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E3
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190808-plasmid-map-sequence-id-372853

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  20. FUGW-APOE-E4-T2A-mCherry

    Plasmid
    #190809
    Purpose
    Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E4
    Promoter
    Ubi C
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190809-plasmid-map-sequence-id-374463

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  21. FUGW-APOE-E2-T2A-mCherry

    Plasmid
    #190807
    Purpose
    Express APOE E2 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E2
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190807-plasmid-map-sequence-id-372851

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  22. FUGW-APOE-E4-T2A-Puro

    Plasmid
    #190810
    Purpose
    Express APOE E4 allele from Ubiquitin C promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E4
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190810-plasmid-map-sequence-id-372856

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  23. pLV floxed hMyoD-IRES-dsRed

    Plasmid
    #66632
    Purpose
    co-expresses CRE excisable human MyoD and dsRed
    Depositor
    Charles Gersbach
    Insert
    human MyoD IRES dsRed (MYOD1 Human)
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hUbC
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66632-plasmid-map-sequence-id-113950

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  24. FUGW-APPwt-T2A-mCherry

    Plasmid
    #190803
    Purpose
    Express wild-type APP from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Ubi C
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190803-plasmid-map-sequence-id-374464

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  27. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid
    #107107
    Purpose
    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.
    Depositor
    Michael E. Wright
    Insert
    human ubiquitin UBB (UBB Human)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Tet
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    107107-plasmid-map-sequence-id-218027

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  28. pJPF0117

    Plasmid
    #130267
    Purpose
    Lentiviral vector for the co-expression of cytoplasmic bPAC and nuclear tagBFP
    Depositor
    Hana El-Samad
    Article
    Fonseca et al bioRxiv 2020, 747030
    Insert
    bPAC
    Use
    Lentiviral
    Tags
    tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    130267-plasmid-map-sequence-id-254858

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  29. ACE2 g2

    Plasmid
    #153012
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153012-plasmid-map-sequence-id-298678

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  30. 6C1A (MBD2)

    Plasmid
    #119958
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD2 (MBD2 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119958-plasmid-map-sequence-id-232631

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  31. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  32. FUGW-APPIce-T2A-mCherry

    Plasmid
    #190806
    Purpose
    Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Icelandic (A673T)
    Promoter
    Ubi C
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    190806-plasmid-map-sequence-id-372850

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  34. pTet-O-APOE-E4-T2A-Puro

    Plasmid
    #190813
    Purpose
    Express APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E4
    Promoter
    Tet-O
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190813-plasmid-map-sequence-id-372861

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  35. pTet-O-APOE-E3-T2A-Puro

    Plasmid
    #190812
    Purpose
    Express APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E3
    Promoter
    Tet-O
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190812-plasmid-map-sequence-id-372860

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  36. pTet-O-APOE-E2-T2A-Puro

    Plasmid
    #190811
    Purpose
    Express APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E2
    Promoter
    Tet-O
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190811-plasmid-map-sequence-id-372859

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  40. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

    Plasmid
    #191528
    Purpose
    Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain
    Depositor
    Yannick Doyon
    Insert
    SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191528-plasmid-map-sequence-id-378116

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  41. FUGW-APPSwe-T2A-Hygro

    Plasmid
    #190805
    Purpose
    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance gene
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Swedish (KM670/671NL)
    Promoter
    Ubi C
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    190805-plasmid-map-sequence-id-372847

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  44. pSFV3

    Plasmid
    #92072
    Purpose
    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Type
    Empty backbone
    Use
    Empty expression plasmid, for run-off transcripti…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92072-plasmid-map-sequence-id-181621

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Showing: 1101 - 1150 of 2028 results