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pLenti-3xFLAG-Puro PAK5 E144K

Plasmid

#167193

Purpose

puromycin resistant lentiviral vector for expression of PAK5 E144K

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 E144K

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 M173I

Plasmid

#167192

Purpose

puromycin resistant lentiviral vector for expression of PAK5 M173I

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 M173I

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CCND1-Puro

Plasmid

#172643

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Flag-Avi-ires-CHD5

Plasmid

#68878

Purpose

Transient mammalian expression of CHD5 for BirA tagging

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG/AVI

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
1438 pSG5L Flag HA PTEN G129R

Plasmid

#10775

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

G129R

Promoter

Available since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pPB-MPP8-112–858_Flag2-Blast

Plasmid

#175039

Purpose

Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PNKP

Plasmid

#20594

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PNKP (PNKP Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat RP deletion

Plasmid

#32452

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Deleted RP domain (amino acids 203-225 missing); …

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-Dicer-5'-pocket mutant

Plasmid

#51368

Depositor

Narry Kim

Article

Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

Insert

Dicer (DICER1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

5' pocket mutant (R778A, R780A, R811A, H982A…

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MOBKL1A

Plasmid

#20503

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MOBKL1A (MOB1B Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag FASTK

Plasmid

#20485

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

FASTK (FASTK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

Plasmid

#170229

Purpose

Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

RHEB (RHEB Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Q64L

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CDK7

Plasmid

#20452

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK7 (CDK7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pRC/CMV

Plasmid

#8514

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Use

Tags

FLAG and His

Expression

Mammalian

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-FLAG-NEMO-C54/347A

Plasmid

#27268

Depositor

Thomas Gilmore

Article

Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

Insert

NEMO (IKBKG Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Cys54Ala Cys347Ala

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

Plasmid

#169166

Purpose

Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

Insert

3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PRKACG

Plasmid

#20597

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKACG (PRKACG Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

Plasmid

#81206

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CSNK1A1L

Plasmid

#20467

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CSNK1A1L (CSNK1A1L Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAPK14

Plasmid

#20524

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK14 (MAPK14 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only