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CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP (GS)

Plasmid

#179637

Purpose

HCVP-inducible tri-transmembrane SEAP RELEASE with GS linkers

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP

Plasmid

#179638

Purpose

HCVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP (GS)

Plasmid

#179639

Purpose

HCVP-inducible single TM SEAP RELEASE construct with GS linkers

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-hcvs-KKMP

Plasmid

#179641

Purpose

HCVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA

Plasmid

#84274

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Flag-GluN2A

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat

Plasmid

#178871

Purpose

Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

Plasmid

#178870

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 P290L BLAST

Plasmid

#164239

Purpose

lentiviral plasmid for GFP-TREX1 P290L

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

P290L point mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 Y305C BLAST

Plasmid

#164240

Purpose

lentiviral plasmid for GFP-TREX1 Y305C

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Y305C point mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 D272fs BLAST

Plasmid

#164237

Purpose

lentiviral plasmid for GFP-TREX1 D272fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D272 frame shift mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI CMV GFP-TREX1 L287fs BLAST

Plasmid

#164238

Purpose

lentiviral plasmid for GFP-TREX1 L287fs

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

L287 frame shift mutation

Promoter

CMV

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

Plasmid

#169743

Purpose

CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

mScarlet-I

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCMV-tetO2

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ3)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154169

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ2)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154170

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ1)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154171

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

Plasmid

#174138

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

Depositor

Benjamin Kleinstiver

Insert

human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-mScarlet

Expression

Mammalian

Mutation

VRER=D1135V/G1218R/R1335E/T1337R

Promoter

CMV and T7

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

Plasmid

#174139

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

Depositor

Benjamin Kleinstiver

Insert

human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet

Use

In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-mScarlet

Expression

Mammalian

Mutation

VRQR=D1135V/G1218R/R1335Q/T1337R

Promoter

CMV and T7

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59Q, TadA* V106W)

Plasmid

#126496

Purpose

Expresses ABEmax(TadA E59Q, TadA*V106W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59Q TadA* V106W)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59, TadA* V106W)

Plasmid

#126497

Purpose

Expresses ABEmax(TadA E59, TadA*V106W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59 TadA* V106W)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Plasmid

#167478

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Use

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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