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Showing: 11401 - 11450 of 148131 results

  1. pX330-PITCh-LMNB1

    Plasmid
    #207770
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207770-plasmid-map-sequence-id-416199

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  7. LCV2_EGFP

    Plasmid
    #155098
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155098-plasmid-map-sequence-id-307983

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  8. TFORF3059

    Plasmid
    #144535
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOSL1 (FOSL1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144535-plasmid-map-sequence-id-391518

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  9. p5M-hPR-B

    Plasmid
    #89114
    Purpose
    mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker
    Depositor
    Elizabeth Wilson
    Article
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Insert
    human progesterone receptor-B (PGR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 restriction sites deleted in polylinker
    Promoter
    CMV
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    89114-plasmid-map-sequence-id-267772

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  11. pCAGGs-Venus

    Plasmid
    #127346
    Purpose
    Expresses Venus constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Phil Sharp
    Insert
    Venus Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS Promoter
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    127346-plasmid-map-sequence-id-246571

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  17. pcDNA3-GFP-MT2A

    Plasmid
    #11613
    Depositor
    Steven Johnson
    Insert
    metallothionein 2A (MT2A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only
    11613-plasmid-map-sequence-id-365342

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  18. pLenti-C-Myc-DDK-IRES-Puro HEXIM1

    Plasmid
    #200726
    Purpose
    Lentiviral expression of Flag-Tagged human HEXIM1
    Depositor
    Claudio Alarcón
    Insert
    HEXIM1 (HEXIM1 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200726-plasmid-map-sequence-id-401797

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  21. AbVec2.0-IGHG2

    Plasmid
    #99576
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99576-plasmid-map-sequence-id-192590

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  26. OPRD1-DuET

    Plasmid
    #213358
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRD1 (OPRD1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213358-plasmid-map-sequence-id-430880

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  28. dCas9 plasmid

    Plasmid
    #100091
    Purpose
    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3XFlag-NLS-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100091-plasmid-map-sequence-id-197565

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  30. mCherry-Calnexin-N-14

    Plasmid
    #55005
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Calnexin (CANX Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    I474N in Calnexin
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. mCherry-Centrin2-N-10

    Plasmid
    #55018
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Centrin2 (CETN2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55018-plasmid-map-sequence-id-371039

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  37. hFerritinL

    Plasmid
    #122653
    Purpose
    Expression of hFerritinL in bacterial cells
    Depositor
    Trevor Douglas
    Insert
    light chain ferritin, human (FTL Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122653-plasmid-map-sequence-id-237551

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  39. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-215852

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  43. pPROEX-TwStrp-FTO

    Plasmid
    #190265
    Purpose
    Expresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.
    Depositor
    Samie Jaffrey
    Insert
    FTO (FTO Human)
    Use
    Tags
    6xHis and Twin-Streptag
    Expression
    Bacterial
    Mutation
    Promoter
    Trc
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    190265-plasmid-map-sequence-id-371437

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  48. pMXs-IP-mVenus-TOSI

    Plasmid
    #172491
    Purpose
    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Pdcd4 (Pdcd4 Mouse)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    172491-plasmid-map-sequence-id-346537

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Showing: 11401 - 11450 of 148131 results